Reduced activity of the pyruvate dehydrogenase complex but not cytochrome c oxidase is associated with neuronal loss in the striatum following short-term forebrain ischemia

Emad Zaidan, Neil R. Sims

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    Previous studies have identified changes in the activities of the pyruvate dehydrogenase complex (PDHC) and cytochrome c oxidase during early recirculation following short-term cerebral ischemia. However, the relationship of these changes to the delayed selective neuronal loss that develops as a result of short-term ischemia is incompletely defined. The effects of ischemia and recirculation on the activities of these enzymes in the dorsolateral striatum, a region containing many susceptible neurons, and the ischemia-resistant paramedian cortex have been compared. No significant loss of activity of cytochrome c oxidase was seen in either region during the first few hours of recirculation following 30 min of ischemia. A decrease (of 32%) was observed at 24 h in the dorsolateral striatum. However, this probably resulted from changes in the mitochondrial fraction due to advanced neuronal degeneration. By contrast, there was a significant decrease (by 24%) in activity of PDHC at 3 h following a 30-min, but not a 10-min, ischemic period. Only the 30-min ischemic period resulted in extensive delayed neuronal loss. In the paramedian cortex, there was no significant change in PDHC and no neuronal loss following either ischemic period. These results provide strong evidence for a close association between neuronal loss and changes in the activity of PDHC but not cytochrome c oxidase in the dorsolateral striatum.

    Original languageEnglish
    Pages (from-to)23-28
    Number of pages6
    JournalBrain Research
    Volume772
    Issue number1-2
    DOIs
    Publication statusPublished - 24 Oct 1997

    Keywords

    • Cytochrome c oxidase
    • Ischemia
    • Mitochondria
    • Neuronal death
    • Pyruvate dehydrogenase complex
    • Selective vulnerability

    Fingerprint

    Dive into the research topics of 'Reduced activity of the pyruvate dehydrogenase complex but not cytochrome c oxidase is associated with neuronal loss in the striatum following short-term forebrain ischemia'. Together they form a unique fingerprint.

    Cite this