Reference values for 30 common biochemistry analytes across 5 different analyzers in neonates and children 30 days to 18 years of age

HAPPI Kids study team, Susan Matthews

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    BACKGROUND: Age-specific reference intervals (RIs) have been developed for biochemistry analytes in children. However, the ability to interpret results from multiple laboratories for 1 individual is limited. This study reports a head-to-head comparison of reference values and agespecific RIs for 30 biochemistry analytes for children across 5 analyzer types.

    METHODS: Blood was collected from healthy newborns and children 30 days to<18 years of age. Serum aliquots from the same individual were analyzed on 5 analyzer types. Differences in the mean reference values of the analytes by the analyzer types were investigated using mixed-effect regression analysis and by comparing maximum variation between analyzers with analyte-specific allowable total error reported in the Westgard QC database. Quantile regression was used to estimate agespecific RIs using power variables in age selected by fractional polynomial regression for the mean, with modification by sex when appropriate.

    RESULTS: The variations of age-specific mean reference values between analyzer types were within allowable total error (Westgard QC) for most analytes, and common age-specific reference limits were reported as functions of age and/or sex. Analyzer-specific reference limits for all analytes on 5 analyzer types are also reported as functions of age and/or sex.

    CONCLUSIONS: This study provides quantitative and qualitative measures of the extent to which results for individual children can or cannot be compared across analyzer types, and the feasibility of RI harmonization. The reported equations enable incorporation of agespecific RIs into laboratory information systems for improving evidence-based clinical decisions in children.

    Original languageEnglish
    Pages (from-to)1317-1326
    Number of pages10
    JournalClinical Chemistry
    Volume65
    Issue number10
    DOIs
    Publication statusPublished - 1 Jan 2019

    Keywords

    • Pediatric Clinical Chemistry
    • biochemistry analytes

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