Regulation of hydrogen production by uncouplering CCCP in green algae chlamydomonas reinhardtii

Chun Qiu Ran, Wei Zhang, Xing Ju Yu, Mei Fang Jin, Mai Cun Deng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Uncouplering carbonylcyanide-m-chlorophenylhydrazone [CCCP] can markedly inhibit the photochemical activity of photosystem II and repress the rate of photosynthetic oxygen evolution. As a result, the anaerobic condition can promptly induce hydrogenase expression and accelerate hydrogen photoproduction under the continuous illumination by Chlamydomonas reinhardtii. When C. reinhardtii cultured in TAP medium and treated with 0, 5 and 15 μmol/L CCCP under the continuous illumination, the photochemical activity of algae could not be obviously inhibited. But 15 and 20 μmol/L CCCP could markedly depress the photochemical activity and substantial amount of hydrogen gas was photoproduced. The photochemical activity of C. reinhardtii cultured with TAP-S at all concentrations of CCCP under the continuous illumination was distinctly inhibited and the cultures photoproduced hydrogen gas rapidly. The algae of C. reinhardtii cultured with TAP and TAP-S medium, different concentrations of CCCP altered the process of hydrogen metabolism and the efficiency of utilization and conversion of solar energy by the center of PS II.

Original languageEnglish
Pages (from-to)62-66
Number of pages5
JournalGaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume27
Issue number1
Publication statusPublished - Jan 2006
Externally publishedYes

Keywords

  • Carbonylcyanide-m-chlorophenylhydrazone (CCCP)
  • Chlamydomonas reinhardtii
  • Hydrogen gas
  • Hydrogenase
  • Photochemical activity

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