Relationship between phenytoin and tolbutamide hydroxylations in human liver microsomes.

CJ Doecke, ME Veronese, SM Pond, JO Miners, DJ Birkett, LN Sansom, ME McManus

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    109 Citations (Scopus)

    Abstract

    1. The metabolic interaction of phenytoin and tolbutamide in human liver microsomes was investigated. 2. Phenytoin 4‐hydroxylation (mean Km 29.6 microM, n = 3) was competitively inhibited by tolbutamide (mean Ki 106.2 microM, n = 3) and tolbutamide methylhydroxylation (mean Km 85.6 microM, n = 3) was competitively inhibited by phenytoin (mean Ki 22.6 microM, n = 3). 3. A significant correlation was obtained between phenytoin and tolbutamide hydroxylations in microsomes from 18 human livers (rs = 0.82, P less than 0.001). 4. Sulphaphenazole was a potent inhibitor of both phenytoin and tolbutamide hydroxylations with IC50 values of 0.4 microM and 0.6 microM, respectively. 5. Mephenytoin was a poor inhibitor of both phenytoin and tolbutamide hydroxylations with IC50 values greater than 400 microM for both reactions. 6. Anti‐rabbit P450IIC3 IgG inhibited both phenytoin and tolbutamide hydroxylations in human liver microsomes by 62 and 68%, respectively. 7. These in vitro studies are consistent with phenytoin 4‐hydroxylation and tolbutamide methylhydroxylation being catalysed by the same cytochrome P450 isozyme(s) in human liver microsomes. 1991 The British Pharmacological Society

    Original languageEnglish
    Pages (from-to)125-130
    Number of pages6
    JournalBritish Journal of Clinical Pharmacology
    Volume31
    Issue number2
    DOIs
    Publication statusPublished - Feb 1991

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