Reliability of Alpha-Defensin 1 Assay in Detecting Periprosthetic Joint Infections: A Pilot Study

Conor Smith, Ashwath Sachchithanantham, Vikas Singh, Christopher Wilson

Research output: Contribution to journalArticlepeer-review

6 Downloads (Pure)

Abstract

Introduction: The intraoperative diagnosis of Periprosthetic Joint Infection (PJI) is a surgical dilemma often requiring sampling
of suspicious tissues for frozen section (FS), deep tissue cultures & histo-pathology (HP). Synovial fluid biomarker Alpha defensin-1
(AD-1) provides rapid intraoperative biomarker testing to assist in detecting PJIs. We aim to study the accuracy of Synovasure TM
in detecting periprosthetic joint infections at our institute.
Methods: 42 patients who underwent revision surgery for suspected periprosthetic joint infections were included in our study
following ethics approval. Synovial fluid from these patients were tested with the Synovasure test kit intraoperatively. Results were
compared with Lab results to compare and assess for false positive and negative results.
Results: 6 patients tested positive with 3 true positives and 3 false positives were also. 36 patients tested negative with 1 false
negative and 35 tree negatives.
Conclusion: No perfect intraoperative test for PJI exists however false negative results are rare using Synovasure had a 97%
specificity and 50% sensitivity rate in detecting periprosthetic joint infections at our institution. This system gives rapid results with
fewer false negatives than conventional frozen section testing.
Original languageEnglish
Article number OAJBS.ID.000442
Pages (from-to)1793-1796
Number of pages4
JournalOpen Access Journal of Biomedical Science
Volume4
Issue number2
DOIs
Publication statusPublished - 27 Apr 2022

Keywords

  • Synovasure
  • Alpha defensin
  • Periprosthetic joint infection
  • Revision
  • Hip
  • Knee
  • Arthroplasty

Fingerprint

Dive into the research topics of 'Reliability of Alpha-Defensin 1 Assay in Detecting Periprosthetic Joint Infections: A Pilot Study'. Together they form a unique fingerprint.

Cite this