Reliable PCR amplifification from Neurospora crassa genomic DNA obtained from conidia

Stephen Henderson; Graham Eariss; David Catcheside

doi:10.4148/1941-4765.1126

Reliable PCR amplifification from Neurospora crassa genomic DNA obtained from conidia

Stephen Henderson
, Graham Eariss
, David Catcheside

Research output: Contribution to journal › Article › peer-review

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Abstract

Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.

Original language	English
Article number	7
Pages (from-to)	24
Number of pages	1
Journal	Fungal Genetics Reports
Volume	52
DOIs	https://doi.org/10.4148/1941-4765.1126
Publication status	Published - 2005

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Final published versionFinal published version, 391 KBLicence: CC BY-SA
Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidiaFinal published version, 381 KBLicence: CC BY-SA

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title = "Reliable PCR amplifification from Neurospora crassa genomic DNA obtained from conidia",

abstract = "Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.",

author = "Stephen Henderson and Graham Eariss and David Catcheside",

year = "2005",

doi = "10.4148/1941-4765.1126",

language = "English",

volume = "52",

pages = "24",

journal = "Fungal Genetics Reports",

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T1 - Reliable PCR amplifification from Neurospora crassa genomic DNA obtained from conidia

AU - Henderson, Stephen

AU - Eariss, Graham

AU - Catcheside, David

PY - 2005

Y1 - 2005

N2 - Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.

AB - Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.

U2 - 10.4148/1941-4765.1126

DO - 10.4148/1941-4765.1126

M3 - Article

VL - 52

SP - 24

JO - Fungal Genetics Reports

JF - Fungal Genetics Reports

M1 - 7

ER -

Reliable PCR amplifification from Neurospora crassa genomic DNA obtained from conidia

Abstract

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