Reply to Bruschi, F.; Casini, G. New Models for the Study of Ocular Toxoplasmosis. Comment on “Ashander et al. Human Retinal Organoid Model of Ocular Toxoplasmosis. Pathogens 2025, 14, 286”

We welcome the comment written by Dr. Bruschi and Dr. Casini regarding our article, “Human retinal organoid model of ocular toxoplasmosis”. This article represented the first report of ocular toxoplasmosis modelled using human retinal organoids. As explained in the Introduction of our article, we sought to demonstrate that infection of retinal organoids with the Apicomplexan parasite, Toxoplasma gondii, was feasible, that this infection progressed over time, and that cells within the organoids responded to the infection. Dr. Bruschi and Dr. Casini have rightly pointed out that it is not possible to ascertain which retinal layers or cell populations are infected with T. gondii in our studies. As noted in the Discussion of our article, we anticipate that future studies by ourselves and other research teams will address this important question...