Response Surface Optimization of Medium Components for Naringinase Production from Staphylococcus xylosus MAK2

Munish Puri, Aneet Kaur, Ram Singh, Anubhav Singh

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Response surface methodology was used to optimize the fermentation medium for enhancing naringinase production by Staphylococcus xylosus. The first step of this process involved the individual adjustment and optimization of various medium components at shake flask level. Sources of carbon (sucrose) and nitrogen (sodium nitrate), as well as an inducer (naringin) and pH levels were all found to be the important factors significantly affecting naringinase production. In the second step, a 22 full factorial central composite design was applied to determine the optimal levels of each of the significant variables. A second-order polynomial was derived by multiple regression analysis on the experimental data. Using this methodology, the optimum values for the critical components were obtained as follows: sucrose, 10.0%; sodium nitrate, 10.0%; pH 5.6; biomass concentration, 1.58%; and naringin, 0.50% (w/v), respectively. Under optimal conditions, the experimental naringinase production was 8.45 U/mL. The determination coefficients (R 2 ) were 0.9908 and 0.9950 for naringinase activity and biomass production, respectively, indicating an adequate degree of reliability in the model.

    Original languageEnglish
    Pages (from-to)181-191
    Number of pages11
    JournalAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
    Volume162
    Issue number1
    DOIs
    Publication statusPublished - 2010

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