@article{46d970d64e524c5db1d535ea3ea1918d,
title = "ROR1 and ROR2 expression in pancreatic cancer",
abstract = "Background: The Wnt receptors ROR1 and ROR2 are generating increased interest as cancer therapeutic targets but remain understudied in pancreatic ductal adenocarcinoma (PDAC). Compared to canonical Wnt/ β-catenin signalling, the role of noncanonical Wnt signalling in PDAC remains largely unknown. Only one study has investigated the prognostic significance of the noncanonical Wnt signalling receptor, ROR2 in PDAC. No studies have investigated the prognostic role of ROR1 in PDAC.Methods: Here, we performed analysis of ROR1 and ROR2 mRNA expression in three publicly available datasets ICGC-PACA-AU (n = 81), TCGA-PAAD (n = 150) and CPTAC-PDAC (n = 137). ROR1 and ROR2 protein expression from the CPTAC-PDAC discovery cohort were also analysed. Immunohistochemistry (IHC) using the validated anti ROR1 monoclonal antibody (4A5) was performed on the Australian Pancreatic Cancer Genome Initiative (APGI) cohort of PDAC samples (n = 152). Association between ROR1 cytoplasmic staining intensity and clinicopathological parameters including stage, grade and overall survival (OS) was investigated.Results: High ROR1 mRNA expression levels correlated with a favourable OS outcome in all of the ICGC-PACA-AU, TCGA-PAAD and CPTAC-PDAC cohorts. ROR1 protein expression was not associated with stage, grade or OS in the APGI cohort.Conclusion: ROR1 and ROR2 have potential as prognostic markers when measured at the mRNA level in PDAC. Our IHC cohort did not support ROR1 protein expression in predicting OS, and highlighted the discrepancy of prognostic biomarkers when measured by MS, IHC and RNAseq.",
keywords = "pancreatic cancer, pancreatic ductal adenocarcinoma, PDAC, ROR1, ROR2, Immunohistochemistry, IHC, Australian Pancreatic Cancer Genome Initiative, APGI",
author = "Dongli Liu and George Sharbeen and Phoebe Phillips and {Australian Pancreatic Cancer Genome Initiative} and Ford, {Caroline E.} and Johns, {Amber L.} and Gill, {Anthony J.} and Chantrill, {Lorraine A.} and Paul Timpson and Angela Chou and Marina Pajic and Tanya Dwarte and David Herrmann and Claire Vennin and Cox, {Thomas R.} and Brooke Pereira and Shona Ritchie and Reed, {Daniel A.} and Chambers, {Cecilia R.} and Xanthe Metcalf and Max Nobis and Nicola Waddell and Pearson, {John V.} and Patch, {Ann Marie} and Katia Nones and Felicity Newell and Pamela Mukhopadhyay and Venkateswar Addala and Stephen Kazakoff and Oliver Holmes and Conrad Leonard and Scott Wood and Grimmond, {Sean M.} and Oliver Hofmann and Samra, {Jaswinder S.} and Nick Pavlakis and Jennifer Arena and High, {Hilda A.} and Ray Asghari and Merrett, {Neil D.} and Amitabha Das and Cosman, {Peter H.} and Kasim Ismail and Alina Stoita and David Williams and Allan Spigellman and Lam, {Vincent W.} and Duncan McLeod and Judy Kirk and Kench, {James G.} and Peter Grimison and Charbel Sandroussi and Annabel Goodwin and Mead, {R. Scott} and Katherine Tucker and Lesley Andrews and Michael Texler and Cindy Forrest and Mo Ballal and David Fletcher and Maria Beilin and Kynan Feeney and Krishna Epari and Sanjay Mukhedkar and Nikolajs Zeps and Nguyen, {Nan Q.} and Ruszkiewicz, {Andrew R.} and Chris Worthley and John Chen and Brooke-Smith, {Mark E.} and Virginia Papangelis and Clouston, {Andrew D.} and Barbour, {Andrew P.} and O{\textquoteright}Rourke, {Thomas J.} and Fawcett, {Jonathan W.} and Kellee Slater and Michael Hatzifotis and Peter Hodgkinson and Mehrdad Nikfarjam and Eshleman, {James R.} and Hruban, {Ralph H.} and Wolfgang, {Christopher L.} and Aldo Scarpa and Lawlor, {Rita T.} and Vincenzo Corbo and Claudio Bassi and Biankin, {Andrew V.} and Jamieson, {Nigel B.} and Chang, {David K.} and Dreyer, {Stephan B.}",
year = "2021",
doi = "10.1186/s12885-021-08952-9",
language = "English",
volume = "21",
journal = "BMC Cancer",
issn = "1471-2407",
publisher = "BioMed Central Ltd.",
}
