SARS-CoV-2 produces a microRNA CoV2-miR-O8 in patients with COVID-19 infection

Elise J. Tucker; Soon Wei Wong; Shashikanth Marri; Saira Ali; Anthony O. Fedele; Michael Z. Michael; Darling Rojas-Canales; Jordan Y. Li; Chuan Kok Lim; Jonathan M. Gleadle

doi:10.1016/j.isci.2023.108719

SARS-CoV-2 produces a microRNA CoV2-miR-O8 in patients with COVID-19 infection

Elise J. Tucker, Soon Wei Wong, Shashikanth Marri, Saira Ali, Anthony O. Fedele, Michael Z. Michael, Darling Rojas-Canales, Jordan Y. Li, Chuan Kok Lim, Jonathan M. Gleadle

College of Medicine and Public Health

Research output: Contribution to journal › Article › peer-review

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Abstract

Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants. CoV2-miR-O8 is highly represented in nasopharyngeal samples from patients with COVID-19 infection, is detected by RT-PCR assays in patients, has features consistent with Dicer and Drosha generation as well as interaction with Argonaute and targets specific human microRNAs.

Original language	English
Article number	108719
Number of pages	13
Journal	iScience
Volume	27
Issue number	1
Early online date	13 Dec 2023
DOIs	https://doi.org/10.1016/j.isci.2023.108719
Publication status	Published - 19 Jan 2024

Keywords

Health sciences
Immunology
Medical specialty
Medicine
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.isci.2023.108719Licence: CC BY-NC-ND

Final published versionFinal published version, 3.32 MBLicence: CC BY-NC-ND

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title = "SARS-CoV-2 produces a microRNA CoV2-miR-O8 in patients with COVID-19 infection",

abstract = "Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants. CoV2-miR-O8 is highly represented in nasopharyngeal samples from patients with COVID-19 infection, is detected by RT-PCR assays in patients, has features consistent with Dicer and Drosha generation as well as interaction with Argonaute and targets specific human microRNAs.",

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AU - Tucker, Elise J.

AU - Wong, Soon Wei

AU - Marri, Shashikanth

AU - Ali, Saira

AU - Fedele, Anthony O.

AU - Michael, Michael Z.

AU - Rojas-Canales, Darling

AU - Li, Jordan Y.

AU - Lim, Chuan Kok

AU - Gleadle, Jonathan M.

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N2 - Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants. CoV2-miR-O8 is highly represented in nasopharyngeal samples from patients with COVID-19 infection, is detected by RT-PCR assays in patients, has features consistent with Dicer and Drosha generation as well as interaction with Argonaute and targets specific human microRNAs.

AB - Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants. CoV2-miR-O8 is highly represented in nasopharyngeal samples from patients with COVID-19 infection, is detected by RT-PCR assays in patients, has features consistent with Dicer and Drosha generation as well as interaction with Argonaute and targets specific human microRNAs.

KW - Health sciences

KW - Immunology

KW - Medical specialty

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SARS-CoV-2 produces a microRNA CoV2-miR-O8 in patients with COVID-19 infection

Abstract

Keywords

UN SDGs

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