Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

Susan N. Christo, Kerrilyn R. Diener, Robert E. Nordon, Michael P. Brown, Hans J. Griesser, Krasimir Vasilev, Farid C. Christo, John D. Hayball

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

Original languageEnglish
Article number7760
Number of pages8
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 14 Jan 2015
Externally publishedYes

Fingerprint

Dive into the research topics of 'Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus'. Together they form a unique fingerprint.

Cite this