Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

Susan N. Christo; Kerrilyn R. Diener; Robert E. Nordon; Michael P. Brown; Hans J. Griesser; Krasimir Vasilev; Farid C. Christo; John D. Hayball

doi:10.1038/srep07760

Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

Susan N. Christo, Kerrilyn R. Diener, Robert E. Nordon, Michael P. Brown, Hans J. Griesser, Krasimir Vasilev, Farid C. Christo, John D. Hayball

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Abstract

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

Original language	English
Article number	7760
Number of pages	8
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep07760
Publication status	Published - 14 Jan 2015
Externally published	Yes

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Christo_Scrutinizing_P2015Final published version, 1.47 MBLicence: CC BY-NC-ND

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abstract = "The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.",

author = "Christo, {Susan N.} and Diener, {Kerrilyn R.} and Nordon, {Robert E.} and Brown, {Michael P.} and Griesser, {Hans J.} and Krasimir Vasilev and Christo, {Farid C.} and Hayball, {John D.}",

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AU - Diener, Kerrilyn R.

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AU - Griesser, Hans J.

AU - Vasilev, Krasimir

AU - Christo, Farid C.

AU - Hayball, John D.

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AB - The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

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Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

Abstract

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