Selective enrichment of omega-3 fatty acids in oils by phospholipase A1

Tushar Moharana, Avinesh Byreddy, Munish Puri, Colin Barrow, Nalam Rao

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    Abstract

    Omega fatty acids are recognized as key nutrients for healthier ageing. Lipases are used to release ω-3 fatty acids from oils for preparing enriched ω-3 fatty acid supplements. However, use of lipases in enrichment of ω-3 fatty acids is limited due to their insufficient specificity for ω-3 fatty acids. In this study use of phospholipase A1 (PLA1), which possesses both sn-1 specific activity on phospholipids and lipase activity, was explored for hydrolysis of ω-3 fatty acids from anchovy oil. Substrate specificity of PLA1 from Thermomyces lenuginosus was initially tested with syntheticp-nitrophenyl esters along with a lipase from Bacillus subtilis (BSL), as a lipase control. Gas chromatographic characterization of the hydrolysate obtained upon treatment of anchovy oil with these enzymes indicated a selective retention of CJ-3 fatty acids in the triglyceride fraction by PLA1 and not by BSL. 13 C NMR spectroscopy based position analysis of fatty acids in enzyme treated and untreated samples indicated that PLA1 preferably retained ω-3 fatty acids in oil, while saturated fatty acids were hydrolysed irrespective of their position. Hydrolysis of structured triglyceride, 1,3-dioleoyl-2-palmitoylglycerol, suggested that both the enzymes hydrolyse the fatty acids at both the positions. The observed discrimination against ω-3 fatty acids by PLA1 appears to be due to its fatty acid selectivity rather than positional specificity. These studies suggest that PLA1 could be used as a potential enzyme for selective concentration of CJ-3 fatty acids.

    Original languageEnglish
    Article numbere0151370
    Pages (from-to)Art: e0151370
    Number of pages14
    JournalPLoS One
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - 2016

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    Moharana, T., Byreddy, A., Puri, M., Barrow, C., & Rao, N. (2016). Selective enrichment of omega-3 fatty acids in oils by phospholipase A1. PLoS One, 11(3), Art: e0151370. [e0151370]. https://doi.org/10.1371/journal.pone.0151370