Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse colon

Ying Hu, Graeme McIntosh, Richard Le Leu, Graeme Young

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Certain forms of dietary Se may have an advantage in improving Se status and reducing cancer risk. The present study compared the effects of an Se-enriched milk protein product (dairy-Se) with an Se yeast (yeast-Se) on selenoprotein activity and expression in the mouse colon. Mice were fed four diets for 4 weeks: a control milk protein diet (Se at 0068 parts per million (ppm)), dairy-Se diets with Se at 05 and 1ppm, and a yeast-Se diet with Se at 1ppm. Cytosolic glutathione peroxidase-1 (GPx-1) activity, mRNA of selenoprotein P (SeP), GPx-1, gastrointestinal glutathione peroxidase-2 (GPx-2) and thioredoxin reductase-1 (TrxR-1) were examined in the mouse colon. Dairy-Se diets did not significantly affect GPx-1 mRNA and GPx-1 activity but produced a dose-dependent increase in SeP and GPx-2 mRNA, with a significantly higher level achieved at 1ppm Se (P<005). Yeast-Se at 1ppm significantly increased GPx-1 mRNA and GPx-1 activity (P<001) but not GPx-2 mRNA. Neither Se supplement had any effect on TrxR-1. The present study indicates that selenoprotein levels in the mouse colon are regulated differently depending on the Se supplement. As we have previously shown that dairy-Se at 1ppm was protective against colorectal cancer (CRC) in an azoxymethane-induced CRC mouse model, this up-regulation of colonic GPx-2 and SeP with Se supplementation may be crucial to its chemopreventive action.

    Original languageEnglish
    Pages (from-to)17-23
    Number of pages7
    JournalBritish Journal of Nutrition
    Volume104
    Issue number1
    DOIs
    Publication statusPublished - Jul 2010

    Keywords

    • Colon cancer prevention
    • Selenium
    • Selenoproteins

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