Separation and Partial Purification of 1,3-β-Glucan and 1,4-β-Glucan Synthases from Saprolegnia

Vincent Bulone, Vincent Girard, Michel Fèvre

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Enriched 1,3-β-glucan and 1,4-β-glucan synthase fractions from the fungus Saprolegnia were isolated by rate zonal centrifugation on glycerol gradient. Purification was improved by entrapment of the enzymes in their reaction product, i.e. microfibrillar glucans. 1,3-β-Glucan syntheses were separated from 1,4-β-glucan syntheses following resuspension of entrapped enzymes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated that 1,3-β-glucan and 1,4-β-glucan syntheses may have a different polypeptide composition because they were enriched for different protein subunits (34, 48, and 50 kD for the 1,3-β-glucan synthase and 60 kD for the 1,4-β-glucan synthase).

Original languageEnglish
Pages (from-to)1748-1755
Number of pages8
JournalPlant Physiology
Volume94
Issue number4
DOIs
Publication statusPublished - Dec 1990
Externally publishedYes

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