TY - JOUR
T1 - Separation and Partial Purification of 1,3-β-Glucan and 1,4-β-Glucan Synthases from Saprolegnia
AU - Bulone, Vincent
AU - Girard, Vincent
AU - Fèvre, Michel
PY - 1990/12
Y1 - 1990/12
N2 - Enriched 1,3-β-glucan and 1,4-β-glucan synthase fractions from the fungus Saprolegnia were isolated by rate zonal centrifugation on glycerol gradient. Purification was improved by entrapment of the enzymes in their reaction product, i.e. microfibrillar glucans. 1,3-β-Glucan syntheses were separated from 1,4-β-glucan syntheses following resuspension of entrapped enzymes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated that 1,3-β-glucan and 1,4-β-glucan syntheses may have a different polypeptide composition because they were enriched for different protein subunits (34, 48, and 50 kD for the 1,3-β-glucan synthase and 60 kD for the 1,4-β-glucan synthase).
AB - Enriched 1,3-β-glucan and 1,4-β-glucan synthase fractions from the fungus Saprolegnia were isolated by rate zonal centrifugation on glycerol gradient. Purification was improved by entrapment of the enzymes in their reaction product, i.e. microfibrillar glucans. 1,3-β-Glucan syntheses were separated from 1,4-β-glucan syntheses following resuspension of entrapped enzymes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated that 1,3-β-glucan and 1,4-β-glucan syntheses may have a different polypeptide composition because they were enriched for different protein subunits (34, 48, and 50 kD for the 1,3-β-glucan synthase and 60 kD for the 1,4-β-glucan synthase).
UR - http://www.scopus.com/inward/record.url?scp=0012001772&partnerID=8YFLogxK
U2 - 10.1104/pp.94.4.1748
DO - 10.1104/pp.94.4.1748
M3 - Article
AN - SCOPUS:0012001772
SN - 0032-0889
VL - 94
SP - 1748
EP - 1755
JO - Plant Physiology
JF - Plant Physiology
IS - 4
ER -