Sequence analysis of a salt tolerant metagenomic clone

Raj Kapardar, Ravi Ranjan, Munish Puri, Rakesh Sharma

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from pro-teobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-depen-dent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.

    Original languageEnglish
    Pages (from-to)212-215
    Number of pages4
    JournalINDIAN JOURNAL OF MICROBIOLOGY
    Volume50
    Issue number2
    DOIs
    Publication statusPublished - 2010

    Keywords

    • ClpS
    • Metagenome
    • Metagenomic
    • Salt tolerance

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