Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction

Dmitriy Khodakov; Anastasia Khodakova; Adrian Linacre; Amanda Ellis

doi:10.1039/c4an00694a

Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.

Original language	English
Pages (from-to)	3548-3551
Number of pages	4
Journal	Analyst
Volume	139
Issue number	14
DOIs	https://doi.org/10.1039/c4an00694a
Publication status	Published - 2014

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abstract = "This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.",

author = "Dmitriy Khodakov and Anastasia Khodakova and Adrian Linacre and Amanda Ellis",

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AU - Khodakova, Anastasia

AU - Linacre, Adrian

AU - Ellis, Amanda

PY - 2014

Y1 - 2014

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AB - This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.

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JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 14

ER -

Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction

Abstract

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