Abstract
This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.
| Original language | English |
|---|---|
| Pages (from-to) | 3548-3551 |
| Number of pages | 4 |
| Journal | Analyst |
| Volume | 139 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2014 |
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Khodakov, D., Khodakova, A., Linacre, A., & Ellis, A. (2014). Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction. Analyst, 139(14), 3548-3551. https://doi.org/10.1039/c4an00694a