Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.

    Original languageEnglish
    Pages (from-to)3548-3551
    Number of pages4
    JournalAnalyst
    Volume139
    Issue number14
    DOIs
    Publication statusPublished - 2014

    Fingerprint Dive into the research topics of 'Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction'. Together they form a unique fingerprint.

  • Cite this