Sequencing at sea: Challenges and experiences in Ion Torrent PGM sequencing during the 2013 Southern Line Islands research expedition

Yan Wei Lim, Daniel A. Cuevas, Genivaldo Gueiros Z. Silva, Kristen Aguinaldo, Elizabeth A. Dinsdale, Andreas F. Haas, Mark Hatay, Savannah E. Sanchez, Linda Wegley-Kelly, Bas E. Dutilh, Timothy T. Harkins, Clarence C. Lee, Warren Tom, Stuart A. Sandin, Jennifer E. Smith, Brian Zgliczynski, Mark J.A. Vermeij, Forest Rohwer, Robert A. Edwards

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Genomics and metagenomics have revolutionized our understanding of marine microbial ecology and the importance of microbes in global geochemical cycles. However, the process of DNA sequencing has always been an abstract extension of the research expedition, completed once the samples were returned to the laboratory. During the 2013 Southern Line Islands Research Expedition, we started the first effort to bring next generation sequencing to some of the most remote locations on our planet. We successfully sequenced twenty six marine microbial genomes, and two marine microbial metagenomes using the Ion Torrent PGM platform on the Merchant Yacht Hanse Explorer. Onboard sequence assembly, annotation, and analysis enabled us to investigate the role of the microbes in the coral reef ecology of these islands and atolls. This analysis identified phosphonate as an important phosphorous source for microbes growing in the Line Islands and reinforced the importance of Lserine in marine microbial ecosystems. Sequencing in the field allowed us to propose hypotheses and conduct experiments and further sampling based on the sequences generated. By eliminating the delay between sampling and sequencing, we enhanced the productivity of the research expedition. By overcoming the hurdles associated with sequencing on a boat in the middle of the Pacific Ocean we proved the flexibility of the sequencing, annotation, and analysis pipelines.

Original languageEnglish
Article numbere520
Number of pages23
JournalPeerJ
Volume2014
Issue number1
DOIs
Publication statusPublished - 19 Aug 2014
Externally publishedYes

Bibliographical note

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

Keywords

  • Coral reef
  • Environmental microbiology
  • Expeditions
  • Genomics
  • Metagenomics
  • Sequencing
  • Vibrio

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