Silver Coating for High-Mass-Accuracy Imaging Mass Spectrometry of Fingerprints on Nanostructured Silicon

Taryn Guinan, Ove Gustafsson, Gordon McPhee, Hilton Kobus, Nicholas Voelcker

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Nanostructure imaging mass spectrometry (NIMS) using porous silicon (pSi) is a key technique for molecular imaging of exogenous and endogenous low molecular weight compounds from fingerprints. However, high-mass-accuracy NIMS can be difficult to achieve as time-of-flight (ToF) mass analyzers, which dominate the field, cannot sufficiently compensate for shifts in measured m/z values. Here, we show internal recalibration using a thin layer of silver (Ag) sputter-coated onto functionalized pSi substrates. NIMS peaks for several previously reported fingerprint components were selected and mass accuracy was compared to theoretical values. Mass accuracy was improved by more than an order of magnitude in several cases. This straightforward method should form part of the standard guidelines for NIMS studies for spatial characterization of small molecules.

    Original languageEnglish
    Pages (from-to)11195-11202
    Number of pages8
    JournalAnalytical Chemistry
    Volume87
    Issue number22
    DOIs
    Publication statusPublished - 2015

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