TY - JOUR
T1 - Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells
AU - Roy Chowdhury, Neelika
AU - Hopp, Isabel
AU - Zilm, Peter
AU - Murray, Patricia
AU - Vasilev, Krasimir
PY - 2018
Y1 - 2018
N2 - In this paper, we interrogate the influence of silver nanoparticle (AgNPs)-based model surfaces on mouse kidney-derived stem cells (mKSCs) differentiation. The widespread use of silver in biomedical and consumer products requires understanding of this element's effect on kidney cells. Moreover, the potential for using stem cells in drug discovery require methods to direct their differentiation to specialized cells. Hence, we generated coated model substrates containing different concentrations of surface immobilized AgNPs, and used them to evaluate properties and functions of mKSCs. Initially, mKSCs exhibited reduced viability on higher silver containing surfaces. However, longer culture periods assisted mKSCs to recover. Greater degree of cell spreading and arborization led by AgNPs, suggest podocyte differentiation. Proximal tubule cell marker's expression revealed differentiation to the specific lineage. Although the exact mechanism underpinning these findings require significant future efforts, this study demonstrate silver's capacity to stimulate mKSC differentiation, which may provide opportunities for drug screenings.
AB - In this paper, we interrogate the influence of silver nanoparticle (AgNPs)-based model surfaces on mouse kidney-derived stem cells (mKSCs) differentiation. The widespread use of silver in biomedical and consumer products requires understanding of this element's effect on kidney cells. Moreover, the potential for using stem cells in drug discovery require methods to direct their differentiation to specialized cells. Hence, we generated coated model substrates containing different concentrations of surface immobilized AgNPs, and used them to evaluate properties and functions of mKSCs. Initially, mKSCs exhibited reduced viability on higher silver containing surfaces. However, longer culture periods assisted mKSCs to recover. Greater degree of cell spreading and arborization led by AgNPs, suggest podocyte differentiation. Proximal tubule cell marker's expression revealed differentiation to the specific lineage. Although the exact mechanism underpinning these findings require significant future efforts, this study demonstrate silver's capacity to stimulate mKSC differentiation, which may provide opportunities for drug screenings.
KW - Silver nanoparticle
KW - stem cells
KW - kidney
KW - Chronic kidney disease
UR - http://www.scopus.com/inward/record.url?scp=85048121540&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/15104212
UR - http://purl.org/au-research/grants/NHMRC/1122825
UR - http://purl.org/au-research/grants/NHMRC/1032738
U2 - 10.1039/c8ra02145g
DO - 10.1039/c8ra02145g
M3 - Article
AN - SCOPUS:85048121540
SN - 2046-2069
VL - 8
SP - 20334
EP - 20340
JO - RSC Advances
JF - RSC Advances
IS - 36
ER -