Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC

Salvatore Sotgia, Dionigia Arru, Elisabetta Sotgiu, Arduino Mangoni, Mauro Forteschi, Gianfranco Pintus, Ciriaco Carru, Angelo Zinellu

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)


    Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.

    Original languageEnglish
    Pages (from-to)945-951
    Number of pages7
    Issue number9
    Publication statusPublished - May 2016


    • amino thione
    • antioxidant
    • assay
    • biomarkers
    • capillary electrophoresis
    • derivatization
    • fluorescence
    • LC
    • low-molecular weight thiols
    • method development


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