Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC

Salvatore Sotgia; Dionigia Arru; Elisabetta Sotgiu; Arduino Mangoni; Mauro Forteschi; Gianfranco Pintus; Ciriaco Carru; Angelo Zinellu

doi:10.4155/bio-2015-0002

Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC

Salvatore Sotgia, Dionigia Arru, Elisabetta Sotgiu, Arduino Mangoni, Mauro Forteschi, Gianfranco Pintus, Ciriaco Carru, Angelo Zinellu

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.

Original language	English
Pages (from-to)	945-951
Number of pages	7
Journal	Bioanalysis
Volume	8
Issue number	9
DOIs	https://doi.org/10.4155/bio-2015-0002
Publication status	Published - May 2016

Keywords

amino thione
antioxidant
assay
biomarkers
capillary electrophoresis
derivatization
fluorescence
LC
low-molecular weight thiols
method development

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@article{eceb0b1204c9459185d484b2a18ae93a,

title = "Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC",

abstract = "Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.",

keywords = "amino thione, antioxidant, assay, biomarkers, capillary electrophoresis, derivatization, fluorescence, LC, low-molecular weight thiols, method development",

author = "Salvatore Sotgia and Dionigia Arru and Elisabetta Sotgiu and Arduino Mangoni and Mauro Forteschi and Gianfranco Pintus and Ciriaco Carru and Angelo Zinellu",

year = "2016",

month = may,

doi = "10.4155/bio-2015-0002",

language = "English",

volume = "8",

pages = "945--951",

journal = "Bioanalysis",

issn = "1757-6199",

publisher = "Future Science",

number = "9",

}

TY - JOUR

T1 - Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC

AU - Sotgia, Salvatore

AU - Arru, Dionigia

AU - Sotgiu, Elisabetta

AU - Mangoni, Arduino

AU - Forteschi, Mauro

AU - Pintus, Gianfranco

AU - Carru, Ciriaco

AU - Zinellu, Angelo

PY - 2016/5

Y1 - 2016/5

N2 - Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.

AB - Background: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. Materials & methods: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 μm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 μm) for CE analysis, monitoring the fluorescence of derivatives. Results: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. Conclusion: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.

KW - amino thione

KW - antioxidant

KW - assay

KW - biomarkers

KW - capillary electrophoresis

KW - derivatization

KW - fluorescence

KW - LC

KW - low-molecular weight thiols

KW - method development

UR - http://www.scopus.com/inward/record.url?scp=84968883661&partnerID=8YFLogxK

U2 - 10.4155/bio-2015-0002

DO - 10.4155/bio-2015-0002

M3 - Article

SN - 1757-6199

VL - 8

SP - 945

EP - 951

JO - Bioanalysis

JF - Bioanalysis

IS - 9

ER -

Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC

Abstract

Keywords

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