Simultaneous fixation and production of catecholamine fluorescence in central nervous tissue by perfusion with aldehydes

J. B. Furness, M. Costa, W. W. Blessing

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    88 Citations (Scopus)

    Abstract

    Perfusion with a mixture of formaldehyde (4%) and glutaraldehyde (0.5%) is shown both to fix central nervous tissue and to produce, with no further treatment, a fluorescence histochemical localization of catecholamines. After perfusion, serial sections can be readily taken through the whole brain with a Vibratome. Landmarks which are apparent at low power with white-light illumination can be seen when the sections are viewed in the fluorescence microscope. Catecholamine-containing nerve cell bodies, varicose axon terminals and non-varicose nerve fibres appear brightly fluorescent and well localized. The method has been applied to rats, guinea-pigs and rabbits and is ideally suited to the accurate mapping of central catecholamine neurons and their processes.

    Original languageEnglish
    Pages (from-to)745-750
    Number of pages6
    JournalThe Histochemical Journal
    Volume9
    Issue number6
    DOIs
    Publication statusPublished - Nov 1977

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