Single-stranded DNA of Tomato leaf curl virus accumulates in the cytoplasm of phloem cells

M. Saif Rasheed, Luke A. Selth, Anna M.G. Koltunow, John W. Randles, M. Ali Rezaian

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Geminiviruses have been reported to replicate in, and localize to, the nuclei of host plant cells. We have investigated the tissue and intracellular distribution of the monopartite Tomato leaf curl virus (TLCV) by in situ hybridization. Contrary to the current understanding of geminiviral localization, single-stranded (ss) DNA of TLCV accumulated in the cytoplasm. TLCV ssDNA was also found in the nucleus, as was lower levels of replicative form double-stranded (ds) DNA. Under the same conditions, Tomato golden mosaic virus (TGMV) ssDNA and dsDNA were found in nuclei. ssDNA of TLCV, TGMV, and Tomato yellow leaf curl Sardinia virus (TYLCSV) was detected in some xylem vessels under specific hybridization conditions. Tissue specificity of TLCV was partially released by co-infection with TGMV. Our observations suggest that the mechanism of TLCV movement may differ from that of bipartite begomoviruses. Crown

Original languageEnglish
Pages (from-to)120-132
Number of pages13
JournalVirology
Volume348
Issue number1
DOIs
Publication statusPublished - 25 Apr 2006
Externally publishedYes

Keywords

  • Begomovirus
  • Cytoplasm
  • Dual color in situ hybridization
  • Geminivirus
  • Phloem-limited virus
  • ssDNA
  • TLCV
  • Xylem

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