Single-stranded DNA of Tomato leaf curl virus accumulates in the cytoplasm of phloem cells

M. Saif Rasheed; Luke A. Selth; Anna M.G. Koltunow; John W. Randles; M. Ali Rezaian

doi:10.1016/j.virol.2005.11.054

Single-stranded DNA of Tomato leaf curl virus accumulates in the cytoplasm of phloem cells

M. Saif Rasheed, Luke A. Selth, Anna M.G. Koltunow, John W. Randles, M. Ali Rezaian

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Geminiviruses have been reported to replicate in, and localize to, the nuclei of host plant cells. We have investigated the tissue and intracellular distribution of the monopartite Tomato leaf curl virus (TLCV) by in situ hybridization. Contrary to the current understanding of geminiviral localization, single-stranded (ss) DNA of TLCV accumulated in the cytoplasm. TLCV ssDNA was also found in the nucleus, as was lower levels of replicative form double-stranded (ds) DNA. Under the same conditions, Tomato golden mosaic virus (TGMV) ssDNA and dsDNA were found in nuclei. ssDNA of TLCV, TGMV, and Tomato yellow leaf curl Sardinia virus (TYLCSV) was detected in some xylem vessels under specific hybridization conditions. Tissue specificity of TLCV was partially released by co-infection with TGMV. Our observations suggest that the mechanism of TLCV movement may differ from that of bipartite begomoviruses. Crown

Original language	English
Pages (from-to)	120-132
Number of pages	13
Journal	Virology
Volume	348
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2005.11.054
Publication status	Published - 25 Apr 2006
Externally published	Yes

Keywords

Begomovirus
Cytoplasm
Dual color in situ hybridization
Geminivirus
Phloem-limited virus
ssDNA
TLCV
Xylem

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title = "Single-stranded DNA of Tomato leaf curl virus accumulates in the cytoplasm of phloem cells",

abstract = "Geminiviruses have been reported to replicate in, and localize to, the nuclei of host plant cells. We have investigated the tissue and intracellular distribution of the monopartite Tomato leaf curl virus (TLCV) by in situ hybridization. Contrary to the current understanding of geminiviral localization, single-stranded (ss) DNA of TLCV accumulated in the cytoplasm. TLCV ssDNA was also found in the nucleus, as was lower levels of replicative form double-stranded (ds) DNA. Under the same conditions, Tomato golden mosaic virus (TGMV) ssDNA and dsDNA were found in nuclei. ssDNA of TLCV, TGMV, and Tomato yellow leaf curl Sardinia virus (TYLCSV) was detected in some xylem vessels under specific hybridization conditions. Tissue specificity of TLCV was partially released by co-infection with TGMV. Our observations suggest that the mechanism of TLCV movement may differ from that of bipartite begomoviruses. Crown",

keywords = "Begomovirus, Cytoplasm, Dual color in situ hybridization, Geminivirus, Phloem-limited virus, ssDNA, TLCV, Xylem",

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AU - Rasheed, M. Saif

AU - Selth, Luke A.

AU - Koltunow, Anna M.G.

AU - Randles, John W.

AU - Rezaian, M. Ali

PY - 2006/4/25

Y1 - 2006/4/25

N2 - Geminiviruses have been reported to replicate in, and localize to, the nuclei of host plant cells. We have investigated the tissue and intracellular distribution of the monopartite Tomato leaf curl virus (TLCV) by in situ hybridization. Contrary to the current understanding of geminiviral localization, single-stranded (ss) DNA of TLCV accumulated in the cytoplasm. TLCV ssDNA was also found in the nucleus, as was lower levels of replicative form double-stranded (ds) DNA. Under the same conditions, Tomato golden mosaic virus (TGMV) ssDNA and dsDNA were found in nuclei. ssDNA of TLCV, TGMV, and Tomato yellow leaf curl Sardinia virus (TYLCSV) was detected in some xylem vessels under specific hybridization conditions. Tissue specificity of TLCV was partially released by co-infection with TGMV. Our observations suggest that the mechanism of TLCV movement may differ from that of bipartite begomoviruses. Crown

AB - Geminiviruses have been reported to replicate in, and localize to, the nuclei of host plant cells. We have investigated the tissue and intracellular distribution of the monopartite Tomato leaf curl virus (TLCV) by in situ hybridization. Contrary to the current understanding of geminiviral localization, single-stranded (ss) DNA of TLCV accumulated in the cytoplasm. TLCV ssDNA was also found in the nucleus, as was lower levels of replicative form double-stranded (ds) DNA. Under the same conditions, Tomato golden mosaic virus (TGMV) ssDNA and dsDNA were found in nuclei. ssDNA of TLCV, TGMV, and Tomato yellow leaf curl Sardinia virus (TYLCSV) was detected in some xylem vessels under specific hybridization conditions. Tissue specificity of TLCV was partially released by co-infection with TGMV. Our observations suggest that the mechanism of TLCV movement may differ from that of bipartite begomoviruses. Crown

KW - Begomovirus

KW - Cytoplasm

KW - Dual color in situ hybridization

KW - Geminivirus

KW - Phloem-limited virus

KW - ssDNA

KW - TLCV

KW - Xylem

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JF - Virology

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ER -

Single-stranded DNA of Tomato leaf curl virus accumulates in the cytoplasm of phloem cells

Abstract

Keywords

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