Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Hasanzadeh M; Alnakhli M; B Delalat; Sinoula Apostolou; FJ Harding; E Mäkilä; JJ Salonen; Bryone Kuss; NH Voelcker

doi:10.1039/c5bm00204d

Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Hasanzadeh M, Alnakhli M, B Delalat, Sinoula Apostolou, FJ Harding, E Mäkilä, JJ Salonen, Bryone Kuss, NH Voelcker

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery.

Original language	English
Pages (from-to)	1555-1565
Number of pages	11
Journal	Biomaterials Science
Volume	3
Issue number	12
DOIs	https://doi.org/10.1039/c5bm00204d
Publication status	Published - 2015

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title = "Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.",

abstract = "In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery.",

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T1 - Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

AU - M, Hasanzadeh

AU - M, Alnakhli

AU - Delalat, B

AU - Apostolou, Sinoula

AU - Harding, FJ

AU - Mäkilä, E

AU - Salonen, JJ

AU - Kuss, Bryone

AU - Voelcker, NH

PY - 2015

Y1 - 2015

N2 - In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery.

AB - In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery.

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Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Abstract

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