Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve

Wolfgang Kummer; Ryuichi Shigemoto; Rainer Haberberger

doi:10.1016/S0304-3940(98)00926-4

Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve

Wolfgang Kummer, Ryuichi Shigemoto, Rainer Haberberger

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The occurrence and distribution of the preferred receptor for the neuropeptide, substance P (SP), the neurokinin-1 receptor (NK1R) was investigated in the vascular supply of the rat sciatic nerve. Messenger RNA for NK1R was demonstrated by RT-PCR in the epineurial layer where the majority of small arteries and arterioles feeding the endoneurial vasculature are located. Immunoreactivity to NK1R-protein was localized on the smooth muscle cells of these arterial vessels by means of immunofluorescence using a polyclonal NK1R antiserum. This muscular localization of NK1R explains the previously reported [Zochodne, D.W. and Ho, L.T., J. Physiol. 444 (1991) 615- 630] moderate vasoconstrictor rather than vasodilator effects of SP in this vascular bed.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	Neuroscience letters
Volume	259
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(98)00926-4
Publication status	Published - 8 Jan 1999
Externally published	Yes

Keywords

Artery
Immunohistochemistry
Peripheral nerve
Substance P
Substance P receptor
Tachykinin receptor
Vasa nervorum

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title = "Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve",

abstract = "The occurrence and distribution of the preferred receptor for the neuropeptide, substance P (SP), the neurokinin-1 receptor (NK1R) was investigated in the vascular supply of the rat sciatic nerve. Messenger RNA for NK1R was demonstrated by RT-PCR in the epineurial layer where the majority of small arteries and arterioles feeding the endoneurial vasculature are located. Immunoreactivity to NK1R-protein was localized on the smooth muscle cells of these arterial vessels by means of immunofluorescence using a polyclonal NK1R antiserum. This muscular localization of NK1R explains the previously reported [Zochodne, D.W. and Ho, L.T., J. Physiol. 444 (1991) 615- 630] moderate vasoconstrictor rather than vasodilator effects of SP in this vascular bed.",

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AU - Shigemoto, Ryuichi

AU - Haberberger, Rainer

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KW - Immunohistochemistry

KW - Peripheral nerve

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KW - Substance P receptor

KW - Tachykinin receptor

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Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve

Abstract

Keywords

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