@inbook{a2b5c8bde54f447c9dd68976c0a3f109,
title = "SNP Genotyping with Amplifluor-Like Method",
abstract = "For SNP genotyping, amplification of fluorescence (Amplifluor) is a popular and actively developing method in the plant sciences. The {"}Amplifluor-like{"} is a {"}home-made{"} modification of the original commercial Amplifluor method. Amplifluor-like genotyping requires two essential components: (1) two allele-specific forward primers targeting the SNP site with one common reverse primer; and (2) a universal part with two non-allele-specific molecular probes containing one of the two used fluorophores and a quencher. Allele discrimination is based on the fluorescence score, where the dominance of one dye over the other confirms the presence of each specific SNP allele. The Amplifluor-like method is similar to commercial KASP and original Amplifluor methods but is much cheaper because all components can be ordered as regular and modified oligos. The easily adaptable Amplifluor-like method can be modified by any researcher to make it suitable for available instruments, reagents and conditions in low-budget laboratories for SNP genotyping of any plant species with identified genetic polymorphism.",
keywords = "Amplifluor-like, Fluorescence, FRET, Genotyping, SNP, Universal molecular probes",
author = "Gulmira Khassanova and Sholpan Khalbayeva and Dauren Serikbay and Shynar Mazkirat and Kulpash Bulatova and Maral Utebayev and Yuri Shavrukov",
year = "2023",
doi = "10.1007/978-1-0716-3024-2_14",
language = "English",
isbn = "9781071630235",
volume = "2638",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer Nature",
pages = "201--219",
editor = "Yuri Shavrukov",
booktitle = "Plant Genotyping",
address = "Germany",
}