Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

Shinji Kihara, Nadine J. Van Der Heijden, Chris K. Seal, Jitendra P. Mata, Andrew E. Whitten, Ingo Köper, Duncan J. McGillivray

    Research output: Contribution to journalArticlepeer-review

    63 Citations (Scopus)


    Upon contact with biological fluids, the surface of nanoparticles is surrounded by many types of proteins, forming a so-called "protein corona". The physicochemical properties of the nanoparticle/corona complex depend predominantly on the nature of the protein corona. An understanding of the structure of the corona and the resulting complex provides insight into the structure-activity relationship. Here, we structurally evaluate the soft and hard components of the protein corona, formed from polystyrene (PS) nanoplastics and human serum albumin (HSA). Using circular dichroism spectroscopy to elucidate the structure of HSA within the complex, we establish the effect of nanoparticle size and pH on the nature of the protein corona formed- whether hard or soft. Despite the weak interaction between PS and the HSA corona, small angle neutron scattering revealed the formation of a complex structure that enhanced the intermolecular interactions between HSA proteins, PS particles, and the HS/PSA complexes. Fractal formation occurred under conditions where the interaction between PS and HSA was strong, and increasing HSA concentrations suppressed the degree of aggregation. The size of the nanoparticles directly influenced the nature of the protein corona, with larger particles favoring the formation of a soft corona, due to the decreased PS-HSA attraction.

    Original languageEnglish
    Pages (from-to)1067-1076
    Number of pages10
    JournalBioconjugate Chemistry
    Issue number4
    Publication statusPublished - 1 Mar 2019


    • nanoparticles
    • protein corona
    • physicochemical


    Dive into the research topics of 'Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona'. Together they form a unique fingerprint.

    Cite this