Somatic embryogenesis in flax

Tejavathi DH; Lakshmi Sita GL; Sunita A. T.

doi:10.1023/A:1006469900025

Somatic embryogenesis in flax

Tejavathi DH, Lakshmi Sita GL, Sunita A. T.

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Callus was initiated from hypocotyl explants of three elite varieties of Linum usitatissimum L. on Murashige and Skoog (MS) medium supplemented with various growth regulators. Somatic embryos differentiated from callus grown on MS + α-naphthaleneacetic acid. The origin from a single cell and subsequent developmental stages was traced. Embryos developed into normal plants upon transfer to MS + 2.69 μM α-naphthaleneacetic acid + 2.22 μM 6-benzyladenine in two varieties. The medium was supplemented with several additives (abscisic acid, glycine, lysine, glutamine and casein hydrolysate) to increase the percentage of normal plant development from embryos; none of the additives could promote the growth of the shoot apex.

Original language	English
Journal	Plant Cell Tissue and Organ Culture
Volume	63
Issue number	2
DOIs	https://doi.org/10.1023/A:1006469900025
Publication status	Published - 1 Nov 2000
Externally published	Yes

Access to Document

10.1023/A:1006469900025Licence: In Copyright

https://rdcu.be/edj17Licence: In Copyright

Cite this

@article{4f3ff36dc4e04630a282ee6fb9058abe,

title = "Somatic embryogenesis in flax",

abstract = "Callus was initiated from hypocotyl explants of three elite varieties of Linum usitatissimum L. on Murashige and Skoog (MS) medium supplemented with various growth regulators. Somatic embryos differentiated from callus grown on MS + α-naphthaleneacetic acid. The origin from a single cell and subsequent developmental stages was traced. Embryos developed into normal plants upon transfer to MS + 2.69 μM α-naphthaleneacetic acid + 2.22 μM 6-benzyladenine in two varieties. The medium was supplemented with several additives (abscisic acid, glycine, lysine, glutamine and casein hydrolysate) to increase the percentage of normal plant development from embryos; none of the additives could promote the growth of the shoot apex.",

author = "Tejavathi DH and GL, {Lakshmi Sita} and {A. T.}, Sunita",

year = "2000",

month = nov,

day = "1",

doi = "10.1023/A:1006469900025",

language = "English",

volume = "63",

journal = "Plant Cell Tissue and Organ Culture",

issn = "0167-6857",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Somatic embryogenesis in flax

AU - DH, Tejavathi

AU - GL, Lakshmi Sita

AU - A. T., Sunita

PY - 2000/11/1

Y1 - 2000/11/1

N2 - Callus was initiated from hypocotyl explants of three elite varieties of Linum usitatissimum L. on Murashige and Skoog (MS) medium supplemented with various growth regulators. Somatic embryos differentiated from callus grown on MS + α-naphthaleneacetic acid. The origin from a single cell and subsequent developmental stages was traced. Embryos developed into normal plants upon transfer to MS + 2.69 μM α-naphthaleneacetic acid + 2.22 μM 6-benzyladenine in two varieties. The medium was supplemented with several additives (abscisic acid, glycine, lysine, glutamine and casein hydrolysate) to increase the percentage of normal plant development from embryos; none of the additives could promote the growth of the shoot apex.

AB - Callus was initiated from hypocotyl explants of three elite varieties of Linum usitatissimum L. on Murashige and Skoog (MS) medium supplemented with various growth regulators. Somatic embryos differentiated from callus grown on MS + α-naphthaleneacetic acid. The origin from a single cell and subsequent developmental stages was traced. Embryos developed into normal plants upon transfer to MS + 2.69 μM α-naphthaleneacetic acid + 2.22 μM 6-benzyladenine in two varieties. The medium was supplemented with several additives (abscisic acid, glycine, lysine, glutamine and casein hydrolysate) to increase the percentage of normal plant development from embryos; none of the additives could promote the growth of the shoot apex.

U2 - 10.1023/A:1006469900025

DO - 10.1023/A:1006469900025

M3 - Article

SN - 0167-6857

VL - 63

JO - Plant Cell Tissue and Organ Culture

JF - Plant Cell Tissue and Organ Culture

IS - 2

ER -

Somatic embryogenesis in flax

Abstract

Access to Document

Fingerprint

Cite this