Sparse feature selection methods identify unexpected global cellular response to strontium-containing materials

Helene Autefage, Eileen Gentleman, Elena Littmann, Martin Hedegaard, Thomas Von Erlach, Matthew O'Donnell, F Burden, David Alan Winkler, M Stevens

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)


    Despite the increasing sophistication of biomaterials design and functional characterization studies, little is known regarding cells' global response to biomaterials. Here, we combined nontargeted holistic biological and physical science techniques to evaluate how simple strontium ion incorporation within the well-described biomaterial 45S5 bioactive glass (BG) influences the global response of human mesenchymal stem cells. Our objective analyses of whole gene-expression profiles, confirmed by standard molecular biology techniques, revealed that strontium-substituted BG up-regulated the isoprenoid pathway, suggesting an influence on both sterol metabolite synthesis and protein prenylation processes. This up-regulation was accompanied by increases in cellular and membrane cholesterol and lipid raft contents as determined by Raman spectroscopy mapping and total internal reflection fluorescence microscopy analyses and by an increase in cellular content of phosphorylated myosin II light chain. Our unexpected findings of this strong metabolic pathway regulation as a response to biomaterial composition highlight the benefits of discovery-driven nonreductionist approaches to gain a deeper understanding of global cell-material interactions and suggest alternative research routes for evaluating biomaterials to improve their design.

    Original languageEnglish
    Pages (from-to)4280-4285
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Issue number14
    Publication statusPublished - 7 Apr 2015


    • Human mesenchymal stem cells
    • Mevalonate pathway
    • Microarray analysis
    • Sparse feature selection analysis
    • Strontium-releasing biomaterials


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