Spectral properties of the light rise of the electro-oculogram

Garima Kapoor, Tran Le Dieu, Sophy Kyi, Hong Hoang, Fiona Tiong, Sandi Trinh, Paul Constable

Research output: Contribution to conferencePaper


Purpose: To examine the light-rise amplitude as a function of
wavelength to determine the optimal wavelength to elicit a maximal
light peak:dark trough ratio (LP:DTratio).
Methods: Electro-oculograms (EOGs) were recorded in 13 subjects
(age 20.9–25.8 years) with recruitment ongoing. Participants’ pupils
were dilated and EOGs were recorded from each eye using gold cup
electrodes with impedance\5 kX using the Veris Science ver 6.48
software and Fresnel ganzfeld (EDI Inc, Miltipas, CA, USA). Participants
performed horizontal 308 saccades every sec over 37 9 10 s
recording epochs every 50 s sampling at 200 Hz and filtered between
0.1 and 30 Hz and 2 k gain. The first two recordings were with white
light generated by LEDs at 30 cd.m-2 followed by 15 epochs under
dark conditions followed by a further 20 epochs at a randomized color
of either white at 100 cd.m-2 or at 30 cd.m-2 with a dominant
wavelength (kd) of 453, 483, 512, 538, 590, or 625 nm. LP:DTratio
maxima for the kd was fitted using the average of both eyes and a
three parameter gaussian function y = ae^{[-0.5(x - xa)/b]2}, with
a equal to the peak of the gaussian, xa to the peak wavelength, and
b the width of the gaussian. A paired Student’s t-test was performed
on the LP:DTratio for each kd to determine the greatist agreement
with the ISCEV white stimulus for the participants. Luminance series
at 483 and 538 nm were performed at 20, 40, 60, 80, and 100 cd.m-2
on a separate day for five of the participants. The fitted log-linear
regression line to the LP:DTratio was compared to the participant’s
ISCEV LP:DTratios to estimate the equivalent luminance required to
match the ISCEV LP:DTratio.
Results: The fitted peak wavelength at which the LP:DTratio reached
its maxima was 501 ± 17 nm with a LP:DTratio of 2.43 ± 0.03.
There was least difference between the cyan (483 nm) 30 cd.m-2
source and the ISCEV standard for the LP:DT ratio 0.08 ± 0.17
[t(12,1), p = 0.654]. The corresponding luminance to reach the
equivalent LP:DTratio for 100 cd.m-2 was obtained with kd = 538
nm at 80 cd.m-2 and 100 cd.m-2 for 483 nm.
Conclusion: The LP:DTratio showed greatest sensitivity to a kd of
501 nm. At a luminance of 80 cd.m-2 using a cyan-green source may
provide an equivalent stimulus at lower luminances than the current
white 100 cd.m-2 ISCEV standard.
Original languageEnglish
Number of pages1
Publication statusPublished - 10 Oct 2019
Event57th Annual Symposium of the International Society for Clinical Electrophysiology of Vision (ISCEV 2019), Seoul, Korea, 7–10 October 2019 - South Korea, Seoul, Korea, Republic of
Duration: 7 Oct 201910 Oct 2019


Conference57th Annual Symposium of the International Society for Clinical Electrophysiology of Vision (ISCEV 2019), Seoul, Korea, 7–10 October 2019
Country/TerritoryKorea, Republic of
Internet address


  • Electro-oculogram (EOG)
  • light
  • spectral properties


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