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Spectral properties of the light rise of the electro-oculogram

  • Garima Kapoor
  • , Tran Le Dieu
  • , Sophy Kyi
  • , Hong Hoang
  • , Fiona Tiong
  • , Sandi Trinh
  • , Paul Constable

    Research output: Contribution to conference › Paper

    Abstract

    Purpose: To examine the light-rise amplitude as a function of
    wavelength to determine the optimal wavelength to elicit a maximal
    light peak:dark trough ratio (LP:DTratio).
    Methods: Electro-oculograms (EOGs) were recorded in 13 subjects
    (age 20.9–25.8 years) with recruitment ongoing. Participants’ pupils
    were dilated and EOGs were recorded from each eye using gold cup
    electrodes with impedance\5 kX using the Veris Science ver 6.48
    software and Fresnel ganzfeld (EDI Inc, Miltipas, CA, USA). Participants
    performed horizontal 308 saccades every sec over 37 9 10 s
    recording epochs every 50 s sampling at 200 Hz and filtered between
    0.1 and 30 Hz and 2 k gain. The first two recordings were with white
    light generated by LEDs at 30 cd.m-2 followed by 15 epochs under
    dark conditions followed by a further 20 epochs at a randomized color
    of either white at 100 cd.m-2 or at 30 cd.m-2 with a dominant
    wavelength (kd) of 453, 483, 512, 538, 590, or 625 nm. LP:DTratio
    maxima for the kd was fitted using the average of both eyes and a
    three parameter gaussian function y = ae^{[-0.5(x - xa)/b]2}, with
    a equal to the peak of the gaussian, xa to the peak wavelength, and
    b the width of the gaussian. A paired Student’s t-test was performed
    on the LP:DTratio for each kd to determine the greatist agreement
    with the ISCEV white stimulus for the participants. Luminance series
    at 483 and 538 nm were performed at 20, 40, 60, 80, and 100 cd.m-2
    on a separate day for five of the participants. The fitted log-linear
    regression line to the LP:DTratio was compared to the participant’s
    ISCEV LP:DTratios to estimate the equivalent luminance required to
    match the ISCEV LP:DTratio.
    Results: The fitted peak wavelength at which the LP:DTratio reached
    its maxima was 501 ± 17 nm with a LP:DTratio of 2.43 ± 0.03.
    There was least difference between the cyan (483 nm) 30 cd.m-2
    source and the ISCEV standard for the LP:DT ratio 0.08 ± 0.17
    [t(12,1), p = 0.654]. The corresponding luminance to reach the
    equivalent LP:DTratio for 100 cd.m-2 was obtained with kd = 538
    nm at 80 cd.m-2 and 100 cd.m-2 for 483 nm.
    Conclusion: The LP:DTratio showed greatest sensitivity to a kd of
    501 nm. At a luminance of 80 cd.m-2 using a cyan-green source may
    provide an equivalent stimulus at lower luminances than the current
    white 100 cd.m-2 ISCEV standard.
    Original languageEnglish
    PagesS3
    Number of pages1
    Publication statusPublished - 10 Oct 2019
    Event57th Annual Symposium of the International Society for Clinical Electrophysiology of Vision (ISCEV 2019), Seoul, Korea, 7–10 October 2019 - South Korea, Seoul, Korea, Republic of
    Duration: 7 Oct 2019 → 10 Oct 2019
    http://iscev2019.com/

    Conference

    Conference57th Annual Symposium of the International Society for Clinical Electrophysiology of Vision (ISCEV 2019), Seoul, Korea, 7–10 October 2019
    Country/TerritoryKorea, Republic of
    CitySeoul
    Period7/10/19 → 10/10/19
    Internet address

    Keywords

    • Electro-oculogram (EOG)
    • light
    • spectral properties

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