Sperm cryopreservation of the noble Scallop Chlamys nobilis by a programmable freezing method: Effect of cryoprotectant

Xing Zheng, Siqi Lin, Rui Yang, Jianguang Qin, Aimin Wang, Zhifeng Gu, Zhenhua Ma

Research output: Contribution to journalArticle

Abstract

A study on Chlamys nobilis sperm cryopreservation by a programmable freezing method was conducted under laboratory condition. Four cryoprotectant agents (dimethyl sulfoxide [DMSO], methanol [MET], propanediol[PG] and ethylene glycol [EG]) and four concentrations (5%, 10%, 20% and 30%) were evaluated for their ability to retain sperm motility, movement characteristics and fertility. Results showed that cryopreserved sperm total motility produced by DMSO and MET at 5%, 10% and 20% were higher than other cryoprotectant treatment groups (CPA groups), as well as rapid sperm percentage. The curvilinear (VCL) and straight line (VSL) velocity produced by DMSO at 5% significantly higher than other CPA groups (p < 0.05), while no significant differences were found for average path (VAP) velocity. The lateral head displacement (ALH) in all CPA groups was similar and without significant difference (p > 0.05), as well as the beat-cross frequency (BCF). A significant higher fertilization rate was produced in DMSO than that in MET at same concentration (p < 0.05), and no significant differences were found for differing concentrations of the same cryoprotectant (p > 0.05). Overall, 5%-20% DMSO was more suitable for Chlamys nobilis sperm programmable cryopreservation when the calcium-free Hanks’ balanced salt solution was used as the extender, and 10°C/min from 0°C to −80°C was used as freezing rate. The findings presented in this study will benefit conservation programs for Chlamys nobilis.

Original languageEnglish
Pages (from-to)1678-1686
Number of pages9
JournalAquaculture Research
Volume50
Issue number6
DOIs
Publication statusPublished - Jun 2019

Keywords

  • cryopreservation
  • cryoprotectant
  • programmable freezing
  • scallop Chlamys nobilis
  • sperm

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