Background: Taxonomic classification is a corner stone for the characterisation and comparison of microbial communities. Currently, most existing methods are either slow, restricted to specific communities, highly sensitive to taxonomic inconsistencies, or limited to genus level classification. As crucial microbiota information is hinging on high-level resolution it is imperative to increase taxonomic resolution to species level wherever possible. Results: In response to this need we developed SPINGO, a flexible and stand-alone software dedicated to high-resolution assignment of sequences to species level using partial 16S rRNA gene sequences from any environment. SPINGO compares favourably to other methods in terms of classification accuracy, and is as fast or faster than those that have higher error rates. As a demonstration of its flexibility for other types of target genes we successfully applied SPINGO also on cpn60 amplicon sequences. Conclusions: SPINGO is an accurate, flexible and fast method for low-level taxonomic assignment. This combination is becoming increasingly important for rapid and accurate processing of amplicon data generated by newer next generation sequencing technologies.
Bibliographical noteFunding Information:
This publication has emanated from research conducted with the financial support of Science Foundation Ireland under Grant Number 11/SIRG/B2162 and SFI/12/RC/2273. Ian B Jeffery is funded under Grant Number 13/SIRG/ 2128. We thank Dr Todd DeSantis for valuable discussions on taxonomic hierarchies.
© 2015 Allard et al.
- 16S rRNA gene amplicons
- Microbiota composition