Stability of the hydrogenase from Tetraselmis subcordiformis and its preliminary purification

Fei Yan, Zhao'an Chen, Xupeng Cao, Hongbin Lu, Song Xue, Wei Zhang

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    1 Citation (Scopus)


    Tetraselmis subcordiformis, a marine green alga, can produce hydrogen by photobiologically hydrolyzing seawater with hydrogenase. In this study, the preliminary purification of the enzyme was explored by ammonium sulfate precipitation, and the impact of sodium dithionite, β-mercaptoethanol and glycerol on the enzyme stability during the process was investigated. The experimental results illustrated that sodium dithionite provided significant protection on the hydrogenase by depleting oxygen, while glycerol, a protectant against the structure instability of the enzyme, also presented protection. Crude enzyme with specific activity of 0.557 U/mg protein was extracted using 60%-70% saturated ammonium sulfate solution supplemented with 200 mmol/L sodium dithionite and 5% glycerol, and the hydrogenase recovery yield was about 30%.

    Original languageEnglish
    Pages (from-to)1003-1008
    Number of pages6
    JournalChinese Journal of Biotechnology
    Issue number7
    Publication statusPublished - Jul 2010


    • Ammonium sulfate precipitation
    • Hydrogenase stability
    • Tetraselmis subcordiformis


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