Statistically quantified measurement of an Alzheimer's marker by surface-enhanced Raman scattering

Ričardas Buividas, Nerijus Dzingelevičius, Reda Kubiliute, Paul R. Stoddart, Vi Khanh Truong, Elena P. Ivanova, Saulius Juodkazis

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Fibrillar forms of the Amyloid-β (Aβ) protein have been implicated in the early stages of Alzheimer's disease (AD), however there are no standardised assays for soluble Aβ oligomer biomarkers that provide the best indication of the disease progression [1,2]. As a step towards a fast and label-free method for testing different AD biomarkers, we have combined laser nano-textured substrates with a SERS mapping technique and validated it using soluble Aβ-40 oligomers [3-5]. The nano-textured SERS substrates provide fast (&5 min), label-free spectra associated with soluble Aβ-40 oligomers down to a concentration of 10 nM. Statistical analysis of the spectral intensities mapped over the substrate surface shows a quantitative correlation with the oligomer concentration. Schematics of experiments: SERS mapping of Aβ-40 (left figure: measured SERS intensity overlayed with an SEM image of ripples) was carried out on the laser nano-textured (ripple) surface of sapphire and statistical analysis of the SERS intensity was carried out for qualitative (a high SERS intensity at low probability) and quantitative (a moderate SERS intenisty at the highest probability) measures. Quantitative statistical analysis of SERS mapping data can be performed off line for cross correlations with other known SERS signatures.

Original languageEnglish
Pages (from-to)567-574
Number of pages8
JournalJournal of Biophotonics
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 2015
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Amyloid-beta
  • Femtosecond laser fabrication
  • Label-free
  • SERS

Fingerprint

Dive into the research topics of 'Statistically quantified measurement of an Alzheimer's marker by surface-enhanced Raman scattering'. Together they form a unique fingerprint.

Cite this