TY - JOUR
T1 - Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide
AU - Dötsch, Jörg
AU - Hänze, Jörg
AU - Dittrich, Katalin
AU - Demirakça, Süha
AU - Haberberger, Rainer
AU - Rascher, Wolfgang
PY - 1997/7/23
Y1 - 1997/7/23
N2 - Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.
AB - Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.
KW - Neuropeptide Y
KW - Nitric oxide (NO) pharmacology
KW - PC12 cells
UR - http://www.scopus.com/inward/record.url?scp=0343051882&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(97)01050-9
DO - 10.1016/S0014-2999(97)01050-9
M3 - Article
C2 - 9274994
AN - SCOPUS:0343051882
SN - 0014-2999
VL - 331
SP - 313
EP - 317
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 2-3
ER -