Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide

Jörg Dötsch, Jörg Hänze, Katalin Dittrich, Süha Demirakça, Rainer Haberberger, Wolfgang Rascher

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10 Citations (Scopus)


Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalEuropean Journal of Pharmacology
Issue number2-3
Publication statusPublished - 23 Jul 1997
Externally publishedYes


  • Neuropeptide Y
  • Nitric oxide (NO) pharmacology
  • PC12 cells


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