Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide

Jörg Dötsch; Jörg Hänze; Katalin Dittrich; Süha Demirakça; Rainer Haberberger; Wolfgang Rascher

doi:10.1016/S0014-2999(97)01050-9

Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide

Jörg Dötsch, Jörg Hänze, Katalin Dittrich, Süha Demirakça, Rainer Haberberger, Wolfgang Rascher

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.

Original language	English
Pages (from-to)	313-317
Number of pages	5
Journal	European Journal of Pharmacology
Volume	331
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-2999(97)01050-9
Publication status	Published - 23 Jul 1997
Externally published	Yes

Keywords

Neuropeptide Y
Nitric oxide (NO) pharmacology
PC12 cells

Access to Document

10.1016/S0014-2999(97)01050-9Licence: In Copyright

Cite this

@article{ff67079ada5c4a19a41a35e0d15d0283,

title = "Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide",

abstract = "Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.",

keywords = "Neuropeptide Y, Nitric oxide (NO) pharmacology, PC12 cells",

author = "J{\"o}rg D{\"o}tsch and J{\"o}rg H{\"a}nze and Katalin Dittrich and S{\"u}ha Demirak{\c c}a and Rainer Haberberger and Wolfgang Rascher",

year = "1997",

month = jul,

day = "23",

doi = "10.1016/S0014-2999(97)01050-9",

language = "English",

volume = "331",

pages = "313--317",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier B.V.",

number = "2-3",

}

TY - JOUR

T1 - Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide

AU - Dötsch, Jörg

AU - Hänze, Jörg

AU - Dittrich, Katalin

AU - Demirakça, Süha

AU - Haberberger, Rainer

AU - Rascher, Wolfgang

PY - 1997/7/23

Y1 - 1997/7/23

N2 - Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.

AB - Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.

KW - Neuropeptide Y

KW - Nitric oxide (NO) pharmacology

KW - PC12 cells

UR - http://www.scopus.com/inward/record.url?scp=0343051882&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(97)01050-9

DO - 10.1016/S0014-2999(97)01050-9

M3 - Article

C2 - 9274994

AN - SCOPUS:0343051882

SN - 0014-2999

VL - 331

SP - 313

EP - 317

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2-3

ER -

Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this