Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas

Long Yu; Changhu Xue; Yaoguang Chang; Xiaoqi Xu; Lei Ge; Guanchen Liu; Yanchao Wang

doi:10.1016/j.foodchem.2013.09.033

Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas

Long Yu, Changhu Xue, Yaoguang Chang, Xiaoqi Xu, Lei Ge, Guanchen Liu, Yanchao Wang

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Thelenota ananas is one of the most popular edible sea cucumber species consumed in China and some Southeast Asian countries. In this study, the delicate structure of fucoidan from T. ananas (Ta-FUC) was clarified. Oligosaccharides and low molecular weight polysaccharides of Ta-FUC were prepared by enzymatic degradation. Their structures, which retained primary structural features of Ta-FUC, were investigated using tandem mass spectrometry and nuclear magnetic resonance. As a result, Ta-FUC was found to be composed of a novel tetrafucose repeating unit [→3-α-L-Fucp-1→3-α-L- Fucp-1→3-α-L-Fucp2,4(OSO3-)-1→3-α-L-Fucp2(OSO3-)-1→]. Meanwhile, it was proven to possess a significant inhibitory effect on superoxide radicals with an IC₅₀ of 17.46 ± 0.14 μg/ml. This effect was related to its sulphation pattern.

Original language	English
Pages (from-to)	113-119
Number of pages	7
Journal	Food Chemistry
Volume	146
DOIs	https://doi.org/10.1016/j.foodchem.2013.09.033
Publication status	Published - 1 Mar 2014
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by National Natural Science Foundation of China (No. 31101302 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110132120015 ), National Natural Science Foundation of China (No. 31101281 ), and Program for Changjiang Scholars and Innovative Research Team in University. The authors sincerely thank Ph.D. Dan Li for proof reading.

Keywords

Fucoidan
Fucoidanase
Mass spectrum
Sea cucumber
Structure
Thelenota ananas

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title = "Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas",

abstract = "Thelenota ananas is one of the most popular edible sea cucumber species consumed in China and some Southeast Asian countries. In this study, the delicate structure of fucoidan from T. ananas (Ta-FUC) was clarified. Oligosaccharides and low molecular weight polysaccharides of Ta-FUC were prepared by enzymatic degradation. Their structures, which retained primary structural features of Ta-FUC, were investigated using tandem mass spectrometry and nuclear magnetic resonance. As a result, Ta-FUC was found to be composed of a novel tetrafucose repeating unit [→3-α-L-Fucp-1→3-α-L- Fucp-1→3-α-L-Fucp2,4(OSO3-)-1→3-α-L-Fucp2(OSO3-)-1→]. Meanwhile, it was proven to possess a significant inhibitory effect on superoxide radicals with an IC50 of 17.46 ± 0.14 μg/ml. This effect was related to its sulphation pattern.",

keywords = "Fucoidan, Fucoidanase, Mass spectrum, Sea cucumber, Structure, Thelenota ananas",

author = "Long Yu and Changhu Xue and Yaoguang Chang and Xiaoqi Xu and Lei Ge and Guanchen Liu and Yanchao Wang",

note = "Funding Information: This work was supported by National Natural Science Foundation of China (No. 31101302 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110132120015 ), National Natural Science Foundation of China (No. 31101281 ), and Program for Changjiang Scholars and Innovative Research Team in University. The authors sincerely thank Ph.D. Dan Li for proof reading.",

year = "2014",

month = mar,

day = "1",

doi = "10.1016/j.foodchem.2013.09.033",

language = "English",

volume = "146",

pages = "113--119",

journal = "Food Chemistry",

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TY - JOUR

T1 - Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas

AU - Yu, Long

AU - Xue, Changhu

AU - Chang, Yaoguang

AU - Xu, Xiaoqi

AU - Ge, Lei

AU - Liu, Guanchen

AU - Wang, Yanchao

N1 - Funding Information: This work was supported by National Natural Science Foundation of China (No. 31101302 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110132120015 ), National Natural Science Foundation of China (No. 31101281 ), and Program for Changjiang Scholars and Innovative Research Team in University. The authors sincerely thank Ph.D. Dan Li for proof reading.

PY - 2014/3/1

Y1 - 2014/3/1

N2 - Thelenota ananas is one of the most popular edible sea cucumber species consumed in China and some Southeast Asian countries. In this study, the delicate structure of fucoidan from T. ananas (Ta-FUC) was clarified. Oligosaccharides and low molecular weight polysaccharides of Ta-FUC were prepared by enzymatic degradation. Their structures, which retained primary structural features of Ta-FUC, were investigated using tandem mass spectrometry and nuclear magnetic resonance. As a result, Ta-FUC was found to be composed of a novel tetrafucose repeating unit [→3-α-L-Fucp-1→3-α-L- Fucp-1→3-α-L-Fucp2,4(OSO3-)-1→3-α-L-Fucp2(OSO3-)-1→]. Meanwhile, it was proven to possess a significant inhibitory effect on superoxide radicals with an IC50 of 17.46 ± 0.14 μg/ml. This effect was related to its sulphation pattern.

AB - Thelenota ananas is one of the most popular edible sea cucumber species consumed in China and some Southeast Asian countries. In this study, the delicate structure of fucoidan from T. ananas (Ta-FUC) was clarified. Oligosaccharides and low molecular weight polysaccharides of Ta-FUC were prepared by enzymatic degradation. Their structures, which retained primary structural features of Ta-FUC, were investigated using tandem mass spectrometry and nuclear magnetic resonance. As a result, Ta-FUC was found to be composed of a novel tetrafucose repeating unit [→3-α-L-Fucp-1→3-α-L- Fucp-1→3-α-L-Fucp2,4(OSO3-)-1→3-α-L-Fucp2(OSO3-)-1→]. Meanwhile, it was proven to possess a significant inhibitory effect on superoxide radicals with an IC50 of 17.46 ± 0.14 μg/ml. This effect was related to its sulphation pattern.

KW - Fucoidan

KW - Fucoidanase

KW - Mass spectrum

KW - Sea cucumber

KW - Structure

KW - Thelenota ananas

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DO - 10.1016/j.foodchem.2013.09.033

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VL - 146

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JF - Food Chemistry

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ER -

Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas

Abstract

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Keywords

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