Studying permeability in a commonly used epithelial cell line: T84 intestinal epithelial cells

Rino Donato, A El-Merhibia, B Gundsambuua, KY Maka, ER Formosaa, X Wang, Catherine Abbott, Barry Powell

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    18 Citations (Scopus)

    Abstract

    The integrity, or barrier function, of the intestinal epithelium is of paramount importance in -maintaining good health. This is largely imparted by a single layer of epithelial cells linked by the transmembrane tight junction protein complex near their apical surface. Disruption of epithelial permeability via the tight junctions can contribute to disease progression. The cytokine IFNγ is involved in many inflammatory processes and has been shown to dramatically increase permeability via changes at the tight junction in experimental models. One of its key effectors is the transcription factor, -IRF-1. In our studies of the role of IRF-1 in barrier function using the human T84 intestinal epithelial cell monolayer model, we have found that induction of IRF-1 alone is insufficient to change permeability and that if IRF-1 is involved in mediating the permeability effects of IFNγ, then other factors must also be required.

    Original languageEnglish
    Title of host publicationPermeability Barrier
    Subtitle of host publicationMethods and Protocols
    EditorsKursad Turksen
    PublisherHUMANA PRESS
    Pages115-137
    Number of pages23
    ISBN (Print)9781617791901
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    Volume763
    ISSN (Print)1064-3745

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