Studying RNA–Protein Interactions In Vivo By RNA Immunoprecipitation

Luke A. Selth, Pierre Close, Jesper Q. Svejstrup

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

19 Citations (Scopus)

Abstract

The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.

Original languageEnglish
Title of host publicationEpigenetics Protocols
EditorsTrygve O. Tollefsbol
PublisherHumana Press Inc.
Pages253-264
Number of pages12
EditionSecond
ISBN (Electronic)978-1-61779-316-5
ISBN (Print)978-1-61779-315-8
DOIs
Publication statusPublished - 24 Oct 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume791
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chromatin immunoprecipitation
  • Gene expression
  • mRNA
  • RNA immunoprecipitation
  • RNA-binding protein
  • Transcription factor

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