Successful expansion of functional and stable regulatory T cells for immunotherapy in liver transplantation

Niloufar Safinia, Trishan Vaikunthanathan, Henrieta Fraser, Sarah Thirkell, Katie Lowe, Laura Blackmore, Gavin Whitehouse, Marc Martinez-Llordella, Wayel Jassem, Alberto Sanchez-Fueyo, Robert I. Lechler, Giovanna Lombardi

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)

Abstract

Strategies to prevent organ transplant rejection whilst minimizing long-term immunosuppression are currently under intense investigation with regulatory T cells (Tregs) nearing clinical application. The clinical trial, ThRIL, recently commenced at King's College London, proposes to use Treg cell therapy to induce tolerance in liver transplant recipients, the success of which has the potential to revolutionize the management of these patients and enable a future of drug-free transplants. This is the first report of the manufacture of clinical grade Tregs from prospective liver transplant recipients via a CliniMACS-based GMP isolation technique and expanded using anti-CD3/CD28 beads, IL-2 and rapamycin. We report the enrichment of a pure, stable population of Tregs (>95% CD4+CD25+FOXP3+), reaching adequate numbers for their clinical application. Our protocol proved successful in, influencing the expansion of superior functional Tregs, as compared to freshly isolated cells, whilst also preventing their conversion to Th17 cells under pro-inflammatory conditions. We conclude with the manufacture of the final Treg product in the clinical research facility (CRF), a prerequisite for the clinical application of these cells. The data presented in this manuscript together with the much-anticipated clinical results from ThRIL, will undoubtedly inform the improved management of the liver transplant recipient.

Original languageEnglish
Pages (from-to)7563-7577
Number of pages15
JournalOncotarget
Volume7
Issue number7
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Immune response
  • Immunity
  • Immunology and microbiology section
  • Immunosuppression
  • Immunotherapy
  • Liver transplantation
  • Regulatory T cells
  • Tolerance

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