The effects of poultry by-product meal (PBM) and selenium yeast (Se-yeast) supplementations on fish meal replacement by soybean meal (SBM) in the diet of golden pompano (Trachinotus ovatus) were examined. In trial I, a 2 × 3 layout including two PBM levels (100 and 170 g kg −1 ) and three levels of fish meal replacement (0, 40 or 60%) was used. At 100 g kg −1 PBM, fish fed the basal diet exhibited the highest weight gain (WG) and nitrogen retention efficiency (NRE) and the lowest feed conversion ratio (FCR). At 170 g kg −1 PBM, no significant differences were found in WG and NRE between fish fed the basal diet and diet with 40% of fish meal replaced by SBM. In trial II, the basal diet containing 170 g kg −1 PBM (trial I) served as a reference. A 2 × 2 layout included two levels of fish meal replacement (40 or 60%) and two levels of Se-yeast addition (0 and 1 g kg −1 ). No significant differences were found in WG, feed intake, FCR and NRE between fish fed the reference diet and diet with 40 or 60% fish meal replacement plus 1 g kg −1 Se-yeast addition. This study indicates that supplementations of PBM and Se-yeast can enhance the level of fish meal replacement by SBM in golden pompano diet. Dietary fish meal level can be reduced to 140 g kg −1 by optimizing inclusion of SBM, PBM and Se-yeast.