TY - JOUR
T1 - Surface chemical functionalities affect the behavior of human adipose-derived stem cells in vitro
AU - Liu, Xujie
AU - Feng, Qingling
AU - Bachhuka, Akash
AU - Vasilev, Krasimir
PY - 2013/4/1
Y1 - 2013/4/1
N2 - This study examines the effect of surface chemical functionalities on the behavior of human adipose-derived stem cells (hASCs) in vitro. Plasma polymerized films rich in amine (NH 2 ), carboxyl (COOH) and methyl (CH 3 ), were generated on hydroxyapatite (HAp) substrates. The surface chemical functionalities were characterized by X-ray photoelectron spectroscopy (XPS). The ability of different substrates to absorb proteins was evaluated. The results showed that substrates modified with hydrophilic functional group (COOH and NH 2 ) can absorb more proteins than these modified with more hydrophobic functional group (CH 3 ). The behavior of human adipose-derived stem cells (hASCs) cultured on different substrates was investigated in vitro: cell counting kit-8 (CCK-8) analysis was used to characterize cell proliferation, scanning electronic microscopy (SEM) analysis was used to characterize cell morphology and alkaline phosphatase (ALP) activity analysis was used to account for differentiation. The results of this study demonstrated that the NH 2 modified surfaces encourage osteogenic differentiation; the COOH modified surfaces promote cell adhesion and spreading and the CH 3 modified surfaces have the lowest ability to induce osteogenic differentiation. These findings confirmed that the surface chemical states of biomaterials can affect the behavior of hASCs in vitro.
AB - This study examines the effect of surface chemical functionalities on the behavior of human adipose-derived stem cells (hASCs) in vitro. Plasma polymerized films rich in amine (NH 2 ), carboxyl (COOH) and methyl (CH 3 ), were generated on hydroxyapatite (HAp) substrates. The surface chemical functionalities were characterized by X-ray photoelectron spectroscopy (XPS). The ability of different substrates to absorb proteins was evaluated. The results showed that substrates modified with hydrophilic functional group (COOH and NH 2 ) can absorb more proteins than these modified with more hydrophobic functional group (CH 3 ). The behavior of human adipose-derived stem cells (hASCs) cultured on different substrates was investigated in vitro: cell counting kit-8 (CCK-8) analysis was used to characterize cell proliferation, scanning electronic microscopy (SEM) analysis was used to characterize cell morphology and alkaline phosphatase (ALP) activity analysis was used to account for differentiation. The results of this study demonstrated that the NH 2 modified surfaces encourage osteogenic differentiation; the COOH modified surfaces promote cell adhesion and spreading and the CH 3 modified surfaces have the lowest ability to induce osteogenic differentiation. These findings confirmed that the surface chemical states of biomaterials can affect the behavior of hASCs in vitro.
KW - Adipose-derived stem cell
KW - Differentiation
KW - Hydroxyapatite
KW - Plasma polymerization
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=84875219320&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2013.01.063
DO - 10.1016/j.apsusc.2013.01.063
M3 - Article
AN - SCOPUS:84875219320
SN - 0169-4332
VL - 270
SP - 473
EP - 479
JO - Applied Surface Science
JF - Applied Surface Science
ER -