Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012

Jana Lai, Heather Cook, Teem-Wing Yip, Jeanette Berthelsen, Stephen Gourley, Victoria Krause, Helen Smith, Amanda Leach, H Smith-Vaughan

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    Abstract

    Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak.Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST).Results: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak.Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age.

    Original languageEnglish
    Article number409
    Number of pages7
    JournalBMC Infectious Diseases
    Volume13
    Issue number409
    DOIs
    Publication statusPublished - 2013

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    Lai, J., Cook, H., Yip, T-W., Berthelsen, J., Gourley, S., Krause, V., Smith, H., Leach, A., & Smith-Vaughan, H. (2013). Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012. BMC Infectious Diseases, 13(409), [409]. https://doi.org/10.1186/1471-2334-13-409