Sustainable remediation - The application of bioremediated soil for use in the degradation of TNT chips

Mason Erkelens, Mohamed Taha Elfouly, Eric Adetutu, Laurelta Tudararo-Aherobo, John Antiabong, Arthur Provatas, Andrew Ball

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    Environmental contamination by TNT (2,4,6 trinitrotoluene), historically used in civilian industries and the military as an explosive is of great concern due to its toxicity. Scientific studies have however shown that TNT is susceptible to microbial transformation. The aim of this study was to assess the potential of a previously bioremediated hydrocarbon contaminated soil (PBR) to increase TNT degradation rates. This was investigated by adding TNT chips to PBR and uncontaminated soils (PNC) in laboratory based studies (up to 16 weeks). Residual TNT chip analysis showed greater TNT degradation in PBR soils (70%) and significantly higher metabolic rates (4.5 fold increase in cumulative CO2 levels) than in PNC soils (30%). Molecular analysis (PCR-DGGE-cluster analysis) showed substantial shifts in soil microbial communities associated with TNT contamination between day 0 and week 4 especially in PBR soils. Bacterial communities appeared to be more sensitive to TNT contamination than fungal communities in both soils. Quantitative PCR analysis showed ~3 fold increase in the abundance of nitroreductase genes (pnrA) in PBR soils with a gradual reduction in community evenness (Pareto-Lorenz curves) in contrast to PNC soils. These results suggest that microbial response to TNT contamination was dependent on the history of soil use. The results also confirm that the microbial potential of waste soils such as PBR soil (usually disposed of via landfill) can be successfully used for accelerated TNT chip degradation. This promotes sustainable re-use of waste soils extending the life span of landfill sites.

    Original languageEnglish
    Pages (from-to)69-76
    Number of pages8
    JournalJournal of Environmental Management
    Volume110
    DOIs
    Publication statusPublished - 15 Nov 2012

    Keywords

    • 2,4,6 Trinitrotoluene
    • Bioremediated soil
    • DGGE
    • Microbial community
    • QPCR

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