Synovial fluid macrophages and blood monocytes differ in their responses to IL-4

P. H. Hart, M. J. Ahern, C. A. Jones, K. L. Jones, M. D. Smith, J. J. Finlay-Jones

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    Abstract

    IL-4 has been described as a potential anti-inflammatory molecule because of its ability in vitro to down-regulate human monocyte production of proinflammatory mediators. In this study, the activity of IL-4 on mononuclear cells and CD14+ macrophages from a site of chronic inflammation, namely, the joints of patients with rheumatoid and psoriatic arthritis, was investigated and compared directly with its activity on PBMC and monocytes from the same patients. In contrast to the response by blood monocytes, the response to IL- 4 by synovial fluid cells was selective; IL-4 did not significantly suppress LPS-induced TNF-α production, but decreased CD14 expression to a similar extent in the two cell populations. IL-4 induction of monocyte/macrophage CD23 expression was investigated as a stimulatory response to IL-4, and although significantly increased on synovial fluid CD14+ cells by IL-4, the induction was considerably reduced compared with that measured for blood cells. Activation and differentiation in vitro of blood monocytes reduced their response to IL-4 for decreased TNF-α production and induction of CD23 and suggested that these biologic phenomena may contribute to the decreased responsiveness to IL-4 by synovial fluid monocytes/macrophages. Thus, IL-4 does not have the same anti-inflammatory properties in vitro on synovial fluid cells as on blood monocytes.

    Original languageEnglish
    Pages (from-to)3370-3380
    Number of pages11
    JournalJournal of Immunology
    Volume151
    Issue number6
    Publication statusPublished - 15 Sep 1993

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