TY - JOUR
T1 - Synthesis and characterization of bifunctional dendrimers: preliminary use for the coating of gold surfaces and the proliferation of human osteoblasts (HOB)
AU - de jong, Edwin
AU - Deloch, Nicole
AU - Knoll, Wolfgang
AU - Turrin, Cedric-Oliver
AU - Majoral, Jean-Pierre
AU - Caminade, Anne-Marie
AU - Koper, Ingo
PY - 2015
Y1 - 2015
N2 - Two different novel families of bifunctional water-soluble dendrimers are synthesized, using the specific functionalization of one function of the cyclotriphosphazene core. Dendrimers are grown from the 5 remaining functions, up to generation 2. Water-solubility is attained in the last step of the synthesis by grafting either ammonium terminal groups or carboxylate terminal groups, on generations 1 and 2 of these bifunctional dendrimers. 12 new compounds are synthesized and fully characterized, in particular by multi-nuclear NMR. The function linked to the core is thioctic acid, suitable for grafting onto gold, thus both types of water-soluble dendrimers can be used to coat gold surfaces. These macromolecular assemblies are characterized by surface plasmon resonance (SPR). In a preliminary attempt, the gold surfaces modified by either positively or negatively charged dendrimers are used for studying their interaction with cells. Exposed to human osteoblast cells (OBC), the influence of the surface coatings on the cell responses is investigated. Polycationic dendrimers provoke cell apoptosis, whereas negatively charged dendrimers support cell adhesion and proliferation.
AB - Two different novel families of bifunctional water-soluble dendrimers are synthesized, using the specific functionalization of one function of the cyclotriphosphazene core. Dendrimers are grown from the 5 remaining functions, up to generation 2. Water-solubility is attained in the last step of the synthesis by grafting either ammonium terminal groups or carboxylate terminal groups, on generations 1 and 2 of these bifunctional dendrimers. 12 new compounds are synthesized and fully characterized, in particular by multi-nuclear NMR. The function linked to the core is thioctic acid, suitable for grafting onto gold, thus both types of water-soluble dendrimers can be used to coat gold surfaces. These macromolecular assemblies are characterized by surface plasmon resonance (SPR). In a preliminary attempt, the gold surfaces modified by either positively or negatively charged dendrimers are used for studying their interaction with cells. Exposed to human osteoblast cells (OBC), the influence of the surface coatings on the cell responses is investigated. Polycationic dendrimers provoke cell apoptosis, whereas negatively charged dendrimers support cell adhesion and proliferation.
UR - http://www.scopus.com/inward/record.url?scp=84940054535&partnerID=8YFLogxK
U2 - 10.1039/c5nj00620a
DO - 10.1039/c5nj00620a
M3 - Article
SN - 1144-0546
VL - 39
SP - 7194
EP - 7205
JO - New Journal of Chemistry
JF - New Journal of Chemistry
IS - 9
ER -