TY - JOUR
T1 - Synthesis, preliminary characterization, and application of novel surfactants from highly branched xyloglucan oligosaccharides
AU - Greffe, Lionel
AU - Bessueille, Laurence
AU - Bulone, Vincent
AU - Brumer, Harry
PY - 2005/4
Y1 - 2005/4
N2 - A novel class of nonionic, carbohydrate-based surfactants has been synthesized from the plant polysaccharide xyloglucan. Enzymatic hydrolysis of xyloglucan yielded a series of well-defined, highly branched oligosaccharides that, following reductive animation, were readily conjugated with fatty acids bearing C8 to C18 chains under mild conditions. The critical micelle concentration, determined by tensiometry and dye-inclusion measurements, showed a typical dependence on acyl chain length and was sensitive to the degree of galactosylation of the head group. Several compounds from this new group of surfactants, especially those with C14 and C 16 chains, were useful for the extraction of membrane-bound enzyme markers from different plant cell compartments in catalytically active form.
AB - A novel class of nonionic, carbohydrate-based surfactants has been synthesized from the plant polysaccharide xyloglucan. Enzymatic hydrolysis of xyloglucan yielded a series of well-defined, highly branched oligosaccharides that, following reductive animation, were readily conjugated with fatty acids bearing C8 to C18 chains under mild conditions. The critical micelle concentration, determined by tensiometry and dye-inclusion measurements, showed a typical dependence on acyl chain length and was sensitive to the degree of galactosylation of the head group. Several compounds from this new group of surfactants, especially those with C14 and C 16 chains, were useful for the extraction of membrane-bound enzyme markers from different plant cell compartments in catalytically active form.
KW - Membrane protein extraction
KW - Reductive animation
KW - Surfactant
KW - Xyloglucan
KW - Xyloglucan oligosaccharides
UR - http://www.scopus.com/inward/record.url?scp=22444442865&partnerID=8YFLogxK
U2 - 10.1093/glycob/cwi013
DO - 10.1093/glycob/cwi013
M3 - Article
C2 - 15537791
AN - SCOPUS:22444442865
SN - 0959-6658
VL - 15
SP - 437
EP - 445
JO - Glycobiology
JF - Glycobiology
IS - 4
ER -