TY - JOUR
T1 - The association between metabolic syndrome severity and oxidative stress induced by maximal exercise testing
T2 - a cross-sectional study
AU - Mallard, Alistair R.
AU - Ramos, Joyce S.
AU - Roberts, Llion A.
AU - Centner, Christoph M.
AU - Fassett, Robert G.
AU - Coombes, Jeff S.
PY - 2019/4/9
Y1 - 2019/4/9
N2 - Purpose: Oxidative stress (OS) has been implicated in the pathogenesis of metabolic syndrome (MetS). The acute change in OS biomarkers due to exercise, known as exercise-induced OS (EIOS), is postulated to be a more appropriate marker of OS compared to spot OS measures. These studies objectives were to investigate EIOS in participants with MetS and compare the associations between EIOS, spot OS measures and MetS severity. Methods: Sixty-three participants with MetS had MetS severity assessed using the MetS Z-score. Participants undertook a cardiorespiratory fitness test (VO2peak) to volitional exhaustion (∼8–12 minutes). Plasma OS (total F2-isoprostanes (IsoP), protein carbonyls (PCs)) and antioxidant (glutathione peroxidase (GPx), total antioxidant status (TAS)) biomarkers were measured from samples obtained before and five minutes post- (Formula presented.) O2peak test. Wilcoxon’s signed-rank tests were used to determine changes in OS markers. Results: There were no significant (p > 0.05) changes in OS or antioxidant biomarkers from pre- to post-exercise (median (interquartile range): IsoP –15.5 (–71.8 to 47.8) pg/mL; PC –0.01 (–0.16 to 0.13) nmol/mg protein; GPx 0.76 (–4.94 to 9.82) U/L, TAS 0.03 (0.00–0.05) mmol/L). Conclusions: A VO2peak test to exhaustion failed to induce OS in participants with MetS. There were no associations between MetS severity and spot OS or EIOS biomarkers.
AB - Purpose: Oxidative stress (OS) has been implicated in the pathogenesis of metabolic syndrome (MetS). The acute change in OS biomarkers due to exercise, known as exercise-induced OS (EIOS), is postulated to be a more appropriate marker of OS compared to spot OS measures. These studies objectives were to investigate EIOS in participants with MetS and compare the associations between EIOS, spot OS measures and MetS severity. Methods: Sixty-three participants with MetS had MetS severity assessed using the MetS Z-score. Participants undertook a cardiorespiratory fitness test (VO2peak) to volitional exhaustion (∼8–12 minutes). Plasma OS (total F2-isoprostanes (IsoP), protein carbonyls (PCs)) and antioxidant (glutathione peroxidase (GPx), total antioxidant status (TAS)) biomarkers were measured from samples obtained before and five minutes post- (Formula presented.) O2peak test. Wilcoxon’s signed-rank tests were used to determine changes in OS markers. Results: There were no significant (p > 0.05) changes in OS or antioxidant biomarkers from pre- to post-exercise (median (interquartile range): IsoP –15.5 (–71.8 to 47.8) pg/mL; PC –0.01 (–0.16 to 0.13) nmol/mg protein; GPx 0.76 (–4.94 to 9.82) U/L, TAS 0.03 (0.00–0.05) mmol/L). Conclusions: A VO2peak test to exhaustion failed to induce OS in participants with MetS. There were no associations between MetS severity and spot OS or EIOS biomarkers.
KW - antioxidants
KW - cardiorespiratory fitness
KW - exercise
KW - F2-isoprostanes
KW - protein carbonyls
UR - http://www.scopus.com/inward/record.url?scp=85064014453&partnerID=8YFLogxK
U2 - 10.1080/1354750X.2019.1600022
DO - 10.1080/1354750X.2019.1600022
M3 - Article
C2 - 30907677
AN - SCOPUS:85064014453
VL - 24
SP - 394
EP - 400
JO - Biomarkers
JF - Biomarkers
SN - 1354-750X
IS - 4
ER -