The cholinergic system in rat testis is of non-neuronal origin

S Schirmer, I Eckhardt, H Lau, J Klein, Yvette DeGraaf, Katrin Lips, C Pineau, Ian Gibbins, Wolfgang Kummer, A Meinhardt, Rainer Haberberger

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    Abstract

    The cholinergic system consists of acetylcholine (ACh), its synthesising enzyme, choline acetyltransferase (CHAT), transporters such as the high-affinity choline transporter (SLC5A7; also known as ChT1), vesicular ACh transporter (SLC18A3; also known as VAChT), organic cation transporters (SLC22s; also known as OCTs), the nicotinic ACh receptors (CHRN; also known as nAChR) and muscarinic ACh receptors. The cholinergic system is not restricted to neurons but plays an important role in the structure and function of non-neuronal tissues such as epithelia and the immune system. Using molecular and immunohistochemical techniques, we show in this study that nonneuronal cells in the parenchyma of rat testis express mRNAs for Chat, Slc18a3, Slc5a7 and Slc22a2 as well as for the CHRN subunits in locations completely lacking any form of innervation, as demonstrated by the absence of protein gene product 9.5 labelling. We found differentially expressed mRNAs for eight α and three β subunits of CHRN in testis. Expression of the α7-subunit of CHRN was widespread in spermatogonia, spermatocytes within seminiferous tubules as well as within Sertoli cells. Spermatogonia and spermatocytes also expressed the α4-subunit of CHRN. The presence of ACh in testicular parenchyma (TP), capsule and isolated germ cells could be demonstrated by HPLC. Taken together, our results reveal the presence of a non-neuronal cholinergic system in rat TP suggesting a potentially important role for non-neuronal ACh and its receptors in germ cell differentiation.

    Original languageEnglish
    Pages (from-to)157-166
    Number of pages10
    JournalReproduction
    Volume142
    Issue number1
    DOIs
    Publication statusPublished - 2011

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  • Cite this

    Schirmer, S., Eckhardt, I., Lau, H., Klein, J., DeGraaf, Y., Lips, K., Pineau, C., Gibbins, I., Kummer, W., Meinhardt, A., & Haberberger, R. (2011). The cholinergic system in rat testis is of non-neuronal origin. Reproduction, 142(1), 157-166. https://doi.org/10.1530/REP-10-0302